Overview

  • Product name

    Human GDF-15 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    1.1 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 104.2 92% - 116%
    Plasma 102.9 88% - 119%
    Cell culture media 114.2 96% - 105%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Product overview

    Abcam's GDF-15 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human GDF-15 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human GDF-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDF-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • GDF-15 measured in cell culture supernatants showing quantity (ng) per mL of tested sample. Samples were diluted 20-540 fold.

  • GDF-15 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 2-54 fold.

Protocols

References

This product has been referenced in:

  • Zhang Y  et al. Sex-specific differences in the modulation of Growth Differentiation Factor 15 (GDF15) by hyperoxia in vivo and in vitro: Role of Hif-1a. Toxicol Appl Pharmacol 332:8-14 (2017). Read more (PubMed: 28734801) »
  • Zhou YM  et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). Read more (PubMed: 26885055) »
See all 2 Publications for this product

Customer reviews and Q&As

nice kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I used human plasma samples for this kit, and it worked well

Abcam user community

Verified customer

Submitted Feb 09 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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