Overview

  • Product name
    Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor)
    See all GDNF kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 119.8 85% - 140%
    Plasma 99.25 86% - 113%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GDNF (Glial Derived Neurotrophic Factor) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human GDNF in plasma and cell culture supernatants. (Human GDNF concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human GDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and GDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human GDNF in normal serum with ab100525, only in serum spiked with Human GDNF.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100525

  • HuGDNF measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100525

Protocols

References

This product has been referenced in:
  • Horibata S  et al. ER-positive breast cancer cells are poised for RET-mediated endocrine resistance. PLoS One 13:e0194023 (2018). Read more (PubMed: 29608602) »
  • Zhang Y  et al. The protein and mRNA expression levels of glial cell line-derived neurotrophic factor in post stroke depression and major depressive disorder. Sci Rep 7:8674 (2017). Read more (PubMed: 28819313) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Excellent Kit

Good Good 4/5 (Ease of Use)
Abreviews
We used the kit to determine GDNF in neuroblastoma cell line culture supernatant. We followed the protocol provided in the kit and we were able to successfully detect the levels of GDNF.

Abcam user community

Verified customer

Submitted Jan 24 2018

Answer


The phenol red within the cell medium should not interfere with the measurement at 450nm but we would suggest running a media control just to make sure which can also act as your negative control.

With regards to the sample preparation, no additional pre-treatment of the customers samples is necessary when using cell supernatant however, it is true that acidifying the samples can enhance the activation of GDNF so if you wish to do this, you can but please remember to add alkali to neutralise the samples.

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Answer

Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have not been tested on rat samples.

We do currently sell antibodies for these targets that would be suitable for use in an ELISA on rat samples:

BDNF:
ab46176 - rabbit polyclonal
ab6201 - rabbit polyclonal

GDNF:
ab28956 - chicken polyclonal

FGF basic: no antibodies tested for ELISA on rat

FGF1:
ab9588 - rabbit polyclonal

I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for your email which has been forwarded to me inthe technical support department.

I am happy to confirm that these kits should not be affected in their performanceif the packagewas not directly placed in therefrigeratorand thatthey have been left a day or two at room temperature.

I have also contacted the laboratory in this regards, and they confirmedas well that the kits are stable for afew days at room temperature:

"The ELISA kits can be stable for several days at room temperature based on our testing. Of course, we strongly recommend storing the kits at 2°C – 8°C upon receipt, but as you said the kit and its components should not have degraded from only one day unrefrigerated..."

Please doalso storethe standardsat -20°C after reconstitutionand avoid repeated freeze/thaw cycles.

I hope this information is helpful.Please do not hesitate to contact us againshould you have any further questions or concerns.

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Answer

Vielen Dank für Ihre Email und Ihren Anruf heute morgen.

Ich kann Ihnen bestätigen, dass beide Kits, ab99978 und ab100525, ohne Problem einen Tag bei Raumtemperatur aushalten können. Die Puffer und Antikörper können dies gut aushalten. Da auch der Standard noch lyophilisiert ist in beiden Kits, mache ich mir auch über den Standard keine Sorgen. Ich kann Ihnen daher empfehlen, diese Kits wie normal zu verwenden.

Ich hoffe dies hilft Ihnen weiter. Bitte zögern Sie nicht, uns wieder zu kontaktieren sollten Sie weitere Fragen oder Bedenken haben.

Ich wünsche Ihnen einen schönen Abend!

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Answer

Thank you for your inquiry.

Unfortunately, we do not know of any publications thatreferenced product at the moment. We will up-date the datasheet assoon as we receive any information about publications using ab100525.

I am sorry to confirm that we cannotdisclose clone numbers of the antibodies in the kits as this proprietary information.

I would like to also re-assure your customer that this kit is covered by our six month Abromise guaranty to work with human cell culture supernatant, urine, serum and plasma.

I apologize that I could not be of more help on this occasion and wish your customer good luck with their experiments.

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