Overview

  • Product name
    Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor)
    See all GDNF kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 119.8 85% - 140%
    Plasma 99.25 86% - 113%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GDNF (Glial Derived Neurotrophic Factor) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human GDNF in plasma and cell culture supernatants. (Human GDNF concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human GDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and GDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human GDNF in normal serum with ab100525, only in serum spiked with Human GDNF.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100525

  • HuGDNF measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100525

Protocols

References

This product has been referenced in:
  • Horibata S  et al. ER-positive breast cancer cells are poised for RET-mediated endocrine resistance. PLoS One 13:e0194023 (2018). Read more (PubMed: 29608602) »
  • Zhang Y  et al. The protein and mRNA expression levels of glial cell line-derived neurotrophic factor in post stroke depression and major depressive disorder. Sci Rep 7:8674 (2017). Read more (PubMed: 28819313) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Ratings

Excellent Kit

Good Good 4/5 (Ease of Use)
Abreviews
We used the kit to determine GDNF in neuroblastoma cell line culture supernatant. We followed the protocol provided in the kit and we were able to successfully detect the levels of GDNF.

Abcam user community

Verified customer

Submitted Jan 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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