Overview

  • Product name

    Human GITR ELISA Kit
    See all GITR kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    MCF7 Extract 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    MCF7 Extract 3 4.4%
  • Sample type

    Serum, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    14 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94 90% - 98%
    Cell culture extracts 92 87% - 96%
    Cell culture media 105 103% - 107%
    Heparin Plasma 95 92% - 101%
    EDTA Plasma 93 90% - 96%
    Citrate Plasma 89 86% - 91%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    GITR in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GITR protein in human serum, plasma, cell culture supernatant and cell extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Glucocorticoid-induced tumor necrosis factor receptor (GITR) is a receptor for GITRL. GITR exists both as a membrane bound receptor protein and a soluble receptor. GITR is expressed mainly on regulatory T-cells (Treg). GITR can induce Treg proliferation, but also inhibits the ability of Treg to suppress the immune response, this can lead to autoimmunity and inflammation.


    Sensitivity:


    Samples in Sample Diluent NS - 18 pg/mL


    Samples in 1X Cell Extraction Buffer PTR - 14 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GITR Capture Antibody 1 x 600µl
    10X Human GITR Detector Antibody 1 x 600µl
    Human GITR Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 4BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • Tissue specificity

    Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
  • Sequence similarities

    Contains 3 TNFR-Cys repeats.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • Activation inducible TNFR family receptor
    • Activation-inducible TNFR family receptor
    • AITR
    • CD357
    • GITR
    • GITR D
    • GITR-D
    • GITRD
    • Glucocorticoid induced TNFR related protein
    • glucocorticoid-induced tnf receptor ligand
    • glucocorticoid-induced tnf receptors ligand
    • Glucocorticoid-induced TNFR-related protein
    • TNF receptor superfamily activation inducible protein
    • TNFRSF 18
    • TNFRSF18
    • TNR18_HUMAN
    • Tumor necrosis factor receptor superfamily member 18
    • Tumor necrosis factor receptor superfamily member 18 precursor
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab234571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of GITR were measured in duplicates, interpolated from the GITR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 25% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of GITR were measured in duplicate and interpolated from the GITR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GITR concentration was determined to be 757 pg/mL in MCF7 cell extract.

Protocols

References

ab234571 has not yet been referenced specifically in any publications.

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