Overview

  • Product name

    Human GLP1 (7-36) ELISA Kit 
    See all GLP1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 8 7%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 9%
  • Sample type

    Cell culture supernatant, Serum, Cell culture media, Hep Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    25 pg/ml
  • Range

    59.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 105% - 112%
    Cell culture media 99 93% - 102%
    Hep Plasma 110 104% - 112%
    Cit plasma 115 107% - 123%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Horse
  • Product overview

    GLP-1(7-36) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GLP-1(7-36) active peptide protein in human serum, plasma and culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    GLP-1 (Glucagon like peptide 1) is part of the group of incretin hormones that are secreted by the gastrointestinal tract in response to food intake to assist glucose stimulated insulin secretion and glucagon suppression. GLP-1 is a 30 aminoacid peptide cleaved from proglucagon and released by the L-cells of the distal ileum. The intracellular precursor of GLP-1 (1-37) is cleaved to form the active peptides GLP-1 (7-37) and GLP-1 (7-36)NH2. The active peptides bind to the GLP-1 receptor (GLP-1r) expressed in the pancreatic beta cell and are quickly metabolized by the enzyme dipeptidyl peptidase IV (DPP-IV) to form the peptide GLP-1 (9-36), which has no insulin stimulating activity. Binding of active GLP-1 to the receptor, increases cAMP levels and potentiates insulin secretion via Protein Kinase A (PKA) and the cAMP-regulated nucleotide exchange factor (Epac2).
    GLP-1 and its receptor are also suggested to play a role in the central nervous systems as mediators of satiety. Intracerebroventricular GLP-1 has been shown to induce c-FOS activity in the hypothalamus and the central nucleus of the amygdala, both of which are important in the regulation of appetite.

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GLP1 (7-36) Capture Antibody 1 x 600µl
    10X Human GLP1 (7-36) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GLP1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
  • Cellular localization

    Secreted.
  • Alternative names

    • GCG
    • Glicentin
    • Glicentin-related polypeptide
    • GLP-1(7-36)
    • GLP-1(7-37)
    • GLP1
    • GLP2
    • Glucagon
    • Glucagon-like peptide 1
    • Glucagon-like peptide 1(7-36)
    • Glucagon-like peptide 1(7-37)
    • Glucagon-like peptide 2
    • GRPP
    • Incretin hormone
    • OXM
    • OXY
    • Oxyntomodulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab184857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay. Serum of each donor was diluted 1/10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

Protocols

References

This product has been referenced in:

  • Kawaguchi T  et al. Effects of isomaltulose on insulin resistance and metabolites in patients with non-alcoholic fatty liver disease: A metabolomic analysis. Mol Med Rep 18:2033-2042 (2018). Read more (PubMed: 29956790) »
  • Zhao L  et al. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus. Ther Clin Risk Manag 13:739-750 (2017). Read more (PubMed: 28721055) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

GLP-1 Human

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The kit was pretty straight forward. We used it for evaluating the expression of this protein in human HepG2 cells treated with several anthocyanins concentrations of colored corn. We found this kit very accurate quantifying this essential marker involved in glucose metabolism.

Abcam user community

Verified customer

Submitted Jun 06 2019

Abreviews
GLUTag cells (in a 6 well plate) were treated with 10uM Forskolin (a known GLP-1 secretagogue) or control media (2ml total volume) for 2 hours.
10ul of cell media was diluted into 40ul of sample diluent (1:5) and subjected to assay. Forskolin caused a significant increase in GLP-1 detection relative to control. n=4-6, P<0.001. data presented as mean +/- SEM.

Dr. Jeffrey Gagnon

Verified customer

Submitted Aug 17 2018

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