Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10 pg/ml
- Range: 0.01 ng/ml - 10 ng/ml
- Sample type: Cell Lysate, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Glucose-6-Phosphate Isomerase ELISA Kit
See all Glucose 6 Phosphate Isomerase kits
Intra-assay Sample n Mean SD CV% HeLa Lysate 9 2% Inter-assay Sample n Mean SD CV% HeLa Lysate 3 2.6%
Sample typeSerum, Cell Lysate, Tissue Homogenate
Assay typeSandwich (quantitative)
Range0.01 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Serum 86 75% - 92% Cell culture media 113 111% - 115% Goat Serum 85 82% - 86% Fetal Bovine Serum 127 121% - 134%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human Glucose-6-phosphate isomerase ELISA kit (ab171575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glucose-6-phosphate isomerase protein in human cell and tissue extracts, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human SIRT1 with 10 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Glucose-6-phosphate isomerase (GPI) is a glycolytic enzyme. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis and is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, resulting in immediate neonatal death and neurological impairment.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Glucose-6-phosphate isomerase Capture Antibody 1 x 600µl 10X Human Glucose-6-phosphate isomerase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4B 2 x 3ml Human Glucose-6-phosphate isomerase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
RelevanceThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Cellular localizationCytoplasmic and Secreted
- Autocrine motility factor
- Glucose phosphate isomerase
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Example GPI standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Titration of HeLa cell lysate within the working range of the assay. Interpolated values from background subtracted data are plotted.
Quantification of GPI expression in different mammalian cell lines. Interpolated values of GPI are plotted for the indicated cell lines based on a lysate concentration of 1 µg/mL.
ab171575 has been referenced in 1 publication.
- Li Y et al. AMF/PGI-mediated tumorigenesis through MAPK-ERK signaling in endometrial carcinoma. Oncotarget 6:26373-87 (2015). PubMed: 26308071