Human Glutathione Peroxidase 1 ELISA Kit (ab193767)

Overview

  • Product name

    Human Glutathione Peroxidase 1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 4.17%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 9.8%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.11 ng/ml
  • Range

    0.4 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 92% - 101%
    Heparin Plasma 99 99% - 100%
    EDTA Plasma 93 91% - 98%
    Citrate Plasma 93 91% - 97%
    Extraction Buffer 96 91% - 100%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Glutathione Peroxidase 1 (GPX1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Glutathione Peroxidase 1 protein in human serum, plasma, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    GPX1 is a selenium-containing enzyme part of the glutathione system responsible for protecting against oxidative damage by catalyzing the reduction of hydroperoxides with glutathione. The homotetramer, GPX1, utilizes its selenocysteine-containing active site to primarily scavenge hydrogen peroxide. The dimerization of glutathione ultimately provides the electrons to reduce the active site, restoring GPX1’s activity. As a critical component of the regulation of oxidative species within cells, GPX1 is directly involved in metabolism, various cancers, and oxidative stress. This assay is specific to isoform 1 of glutathione peroxidase.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GPX1 Capture Antibody 1 x 600µl
    10X Human GPX1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GPX1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Protects the hemoglobin in erythrocytes from oxidative breakdown.
  • Sequence similarities

    Belongs to the glutathione peroxidase family.
  • Post-translational
    modifications

    During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • Cellular glutathione peroxidase
    • Glutathione peroxidase 1
    • GPx-1
    • GPX1
    • GPX1_HUMAN
    • GSHPx-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab193767 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We recommend to store reconstituted standards at -80°C, all other assay components at 4°C, and to keep TMB substrate solution protected from light.

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