Overview

  • Product name

    Human Glycogen Synthase 1 ELISA Kit
    See all Glycogen synthase 1/GYS1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 2.5%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 5.6%
  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    14 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 80 76% - 84%
    Tissue Extracts 108 105% - 112%
    Heparin Plasma 81 77% - 83%
    EDTA Plasma 95 90% - 97%
    Citrate Plasma 89 85% - 92%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Glycogen Synthase 1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Glycogen Synthase 1 protein in human serum, plasma, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Glycogen Synthase 1 (also known as Glycogen [starch] synthase, muscle) is a glycosyltransferase enzyme involved in converting glucose to glycogen. Mutations in Glycogen Synthase 1 are associated with glycogen storage disease. Mouse and rat Glycogen Synthase 1 have 97% protein sequence identity to human Glycogen Synthase 1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab214031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 38 pg/µg HELA extract, 129 pg/µg HEK293 extract and 5.9 pg/µg Liver extract.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Glycogen Synthase 1 were measured in duplicates, interpolated from the Glycogen Synthase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows:  serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 

  • The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 5.7 pg/µg NIH3T3 extract and 595 pg/µg PC12 extract

Protocols

References

ab214031 has not yet been referenced specifically in any publications.

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