Overview

  • Product name
    Human GM-CSF ELISA Kit
    See all GM-CSF kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.05 pg/ml - 500 pg/ml
  • Recovery

    > 75 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.1 89% - 114%
    Serum 75.43 69% - 83%
    Plasma 74.49 68% - 83%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GM-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF in serum, plasma, and cell culture supernatants

    This assay employs an antibody specific for Human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical standard curve
    Typical standard curve

Protocols

References

ab100529 has not yet been referenced specifically in any publications.

Publishing research using ab100529? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First

Product code: 100529
Lot number: GR86435-1
Inquiry: Dear Sir, with attachment the results (OD of standered curve) of my work on GMCSF for 2 kit , please let me know if these results are good so I can evaluate the results of my patients which I ...

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Thank you for contacting us.

I have reviewed the data you have kindly sent and I am assuming that the data is for standard curve. The readings seems not good enough because the OD for 0 is comparable to 2.05pg/ml. The OD for "0" pg/m...

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They are free ! What we should pay??????

Yes, It is all free however they need to check if the items were paid in original order.

It is just a normal check so there is no need to worry, you will be getting a order confirmation soon.

I didn't hear about the order shipping .. etc.. Please let me know ASAP, as the customer waiting !
Truly yours,

Our accountsdepartment is holding the order at the moment. The order will be shipped once they authorize it, after checking thepayment etc. Please email mailto:creditcontrol@abcam.com for any order update.

Thanks!

Please see below:

thank you for your reply,

I have worked the tow kits on the same day as the incubation time between the kits is enough and when I got a step addition of HRP-Streptavidin noted deficiencies and tried to use the second...

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Thank you for contacting us.

To prevent the shortageissue in the future, please advise your customer along with other customers to check all reagents before beginning the experiment and that if there is a volume shortage or any other issue to ...

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thank you for your reply,I have worked the tow kits on the same day as the incubation time between the kits is enough and when I got a step addition of HRP-Streptavidin noted deficiencies and tried to use the second kit to avoid the lack of the first I...

Read More

Thank you for your cooperation.

I have sent a request to lab for new kits and hoping to get an answer later this evening. I will confirm you the dispatch date.

I hope this info will be helpful.

Thanks a lot for your support, it is really appreciated !
Please see below reply from the end user:
Thank you and thanks Abcam for your help and support, kindly remember it was 2 kits of ab100562 cause I have 176 sample to run, I will hoping to...

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Thank you for your email.

Regarding ab100562 have you used both kits? If not, then I will send you one replacement kit and one extra vial of HRP-standard. Could you let me know if you still have to use one kit of ab100562?

There are m...

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Thank you for your prompt reply.

Concerning stock item G, i centrifuged the vial before taking the liquid out, after spinning i recovered only 10µl. The failure of the experiment i expect was dueto the insufficient amount ofHRP-strepativ...

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That's fine!

I will be sending you a replacement kitofab100562.

Could you check the HRP-streptavidin amount in ab100529.Is it OK?

Kindly be informed that you have supplied me recently with two kits ofInterleukin 1 beta Human ELISA (code: ab100562)(lot:GR85228-1)from Abcam Company which were unable to give me any result because of the following:
- The kit should contain 200&m...

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Thank you for your email. I apologize for any inconvenience caused.

The volume 200ul is actually more than what is need for the assay; this extra volume is added to avoid any loss due to evaporation, insufficient centrifuging, etc. Actually on...

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Dear sir,

U told me the range for the product



Product code: 100529, Lot number: GR86435-1 is
2.05 pg/ml - 500 pg/ml ,Is this the normal value if not please provide me with the normal value according to age and gen...

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Thank you for your reply.
I confirm that 2.05 pg/ml - 500 pg/ml is the range the kit detects.
Please consult the appropriate literature for the normal concentration for typical concentration of GM-CSF in healthy humans.
A typical standar...

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Product code: 100529
Lot number: GR86435-1

Inquiry: Good Day, Please i need the normal range for this prosuct to assase product result. thanks and best regards

Thank you for your inquiry.
I am happy to confirm that the range of this kit is typically 2.05 pg/ml - 500 pg/ml as stated on the datasheet.
We also have data on the recovery that can also be found on the datasheet and in the protocol booklet...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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