Product nameHuman GODZ/DHHC-3 peptide
Our Abpromise guarantee covers the use of ab31882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-GODZ/DHHC-3 antibody (ab31837)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Previously labelled as GODZ.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- GABA-A receptor-associated membrane protein 1
FunctionPalmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.
Sequence similaritiesBelongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger.
DomainThe DHHC domain is required for palmitoyltransferase activity.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab31882 has been referenced in 1 publication.
- Mukherjee A et al. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem 292:4152-4163 (2017). PubMed: 28154191