Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (ab257976)
Overview
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Product name
Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate
See all GOLPH2 kits -
Product overview
Knockout cell lysate achieved by CRISPR/Cas9. -
Parental Cell Line
HEK293T -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. -
Passage number
<20 -
Knockout validation
Sanger Sequencing -
Reconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
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Notes
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260409 - Human GOLM1 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Target
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Function
Unknown. Cellular response protein to viral infection. -
Tissue specificity
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. -
Sequence similarities
Belongs to the GOLM1/CASC4 family. -
Post-translational
modificationsGlycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. - Information by UniProt
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Alternative names
- bA379P1.3
- C9orf155
- Chromosome 9 open reading frame 155
see all
Associated products
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KO cell lines
Images
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Lane 1: Wild-type HEK-293T cell lysate, 10 ug
Lane 2: GOLM1 knockout HEK-293T cell lysate, 10 ug
Lane 3: U-2 OS cell lysate, 20 ug
Lane 4: HepG2 cell lysate, 20 ug
Lane 5: MDA-MB-231 cell lysate, 20 ug
Lane 6: LNCaP cell lysate, 20 ug
False colour image of Western blot: Anti-GOLPH2 antibody [OTI6C9] staining at 1/500 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab119800 was shown to bind specifically to GOLPH2. A band was observed at 70-75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GOLM1 knockout cell line ab266505 (knockout cell lysate ab257976). To generate this image, wild-type and GOLM1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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Homozygous: 1 bp insertion in exon 3
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab257976 has not yet been referenced specifically in any publications.