Overview

  • Product name

    Human Granulysin ELISA Kit
    See all GNLY/Granulysin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 355pg/ml 20.59 5.8%
    2 16 862pg/ml 38.79 4.5%
    3 16 1513pg/ml 65 4.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 324pg/ml 26.5 8.2%
    2 24 765pg/ml 55.85 7.3%
    3 24 1322pg/ml 89.8 6.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Granulysin ELISA Kit (ab213787) is designed for the quantitative measurement of Human Granulysin in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Granulysin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-L145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Granulysin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Granulysin amount of sample captured in plate. 

  • Notes

    Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly anti-microbial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Granulysin Coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated Anti-Human Granulysin Antibody 1 x 130µl
    Lyophilized Recombinant Human Granulysin Standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
  • Tissue specificity

    Expressed in natural killer and T-cells.
  • Sequence similarities

    Contains 1 saposin B-type domain.
  • Post-translational
    modifications

    A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.
  • Cellular localization

    Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.
  • Information by UniProt
  • Alternative names

    • 519
    • D2S69E
    • GNLY
    • GNLY_HUMAN
    • Granulysin
    • Granulysin precursor
    • LAG 2
    • LAG2
    • Lymphocyte activation gene 2
    • Lymphokine LAG 2
    • Lymphokine LAG-2
    • NKG 5
    • NKG5
    • Protein NKG5
    • T cell activation protein 519
    • T lymphocyte activation gene 519
    • T-cell activation protein 519
    • TLA519
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Granulysin ELISA Kit (ab213787) Standard Curve

Protocols

References

ab213787 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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