Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

Overview

  • Product name
    Human Growth Factor Antibody Array - Membrane (41 Targets)
    See all Growth Factor Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.


    Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, array ab133998 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab134002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002.

  • Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.

  • Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.

  • Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

Protocols

References

This product has been referenced in:
  • Shiga Y  et al. Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period. Asian Spine J 11:329-336 (2017). Read more (PubMed: 28670400) »
  • Sie M  et al. Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma. PLoS One 10:e0122555 (2015). Human . Read more (PubMed: 25799134) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your interest. The laboratory reports that the anti-human array has not been tested with mouse samples, and there is currently no corresponding anti-mouse growth factor array. However, the anti-mouse cytokine arrays, such as ab169820, share a few of the same targets with the GF arrays. The complete selection can be found by searching for 'mouse cytokine array' on the Abcam website.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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