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  1. Link

    human-growth-factor-antibody-array-membrane-41-targets-ab134002.pdf

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Immunology Innate Immunity Cytokines CSFs
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Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

  • Datasheet
  • SDS
  • Protocol Booklet
Q&A (1)References (13)

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Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
  • Quantification of Human Growth Factor Antibody Array
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
  • Quantification of Human Growth Factor Antibody Array
  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
  • Quantification of Human Growth Factor Antibody Array

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Overview

  • Product name

    Human Growth Factor Antibody Array - Membrane (41 Targets)
    See all Growth Factor Antibody Array antibody arrays
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.


    Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, array ab133998 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Biotin-Conjugated Anti-Cytokines 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 2 x 25ml
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
    Growth Factor Antibody Array Membranes 4 units 8 units
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • CSFs
    • Antibody Arrays
    • Membrane antibody arrays
    • EGFR phosphorylation antibody arrays
    • Antibody Arrays
    • Membrane antibody arrays
    • Growth factor antibody arrays

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection
Use at an assay dependent concentration.
Notes
Multiplex Protein Detection
Use at an assay dependent concentration.

Images

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002.

  • Quantification of Human Growth Factor Antibody Array
    Quantification of Human Growth Factor Antibody Array

    Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.

  • Quantification of Human Growth Factor Antibody Array
    Quantification of Human Growth Factor Antibody Array

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

  • Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
    Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.

  • Quantification of Human Growth Factor Antibody Array
    Quantification of Human Growth Factor Antibody Array

    Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab134002? Please let us know so that we can cite the reference in this datasheet.

ab134002 has been referenced in 13 publications.

  • Pulcini S  et al. Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential. Int J Mol Sci 22:N/A (2021). PubMed: 34445472
  • Satyam A  et al. Cell derived extracellular matrix-rich biomimetic substrate supports podocyte proliferation, differentiation and maintenance of native phenotype. Adv Funct Mater 30:N/A (2020). PubMed: 33692659
  • Van den Bogerd B  et al. Exploring the Mesenchymal Stem Cell Secretome for Corneal Endothelial Proliferation. Stem Cells Int 2020:5891393 (2020). PubMed: 32089707
  • De Kock J  et al. Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells. Cells 9:N/A (2020). PubMed: 32276503
  • Hong S  et al. Self-Organized Liver Microtissue on a Bio-Functional Surface: The Role of Human Adipose-Derived Stromal Cells in Hepatic Function. Int J Mol Sci 21:N/A (2020). PubMed: 32610471
View all Publications for this product

Customer reviews and Q&As

Q&A
Submit a question

Question

Hi, I am looking for a membrane antibody array for growth factors in mouse samples, do you have this type of production? I only found in human from your website.

Read More

Abcam community

Verified customer

Asked on Jul 03 2013

Answer

Thank you for your interest. The laboratory reports that the anti-human array has not been tested with mouse samples, and there is currently no corresponding anti-mouse growth factor array. However, the anti-mouse cytokine arrays, such as ab169820, share a few of the same targets with the GF arrays. The complete selection can be found by searching for 'mouse cytokine array' on the Abcam website.

Read More

Tom Ruyle

Abcam Scientific Support

Answered on Jul 03 2013

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