Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameHuman Growth Factor Antibody Array - Membrane (41 Targets)
See all Growth Factor Antibody Array antibody arrays -
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
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Assay typeSemi-quantitative
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Species reactivityReacts with: Human
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Product overview
ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.
Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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Notes
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Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Growth Factor Antibody Array Membranes 4 units 8 units -
Research areas
Applications
Our Abpromise guarantee covers the use of ab134002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Multiplex Protein Detection | Use at an assay dependent concentration. |
Images
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Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002.
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Quantification of Human Growth Factor Antibody ArrayCells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.
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Quantification of Human Growth Factor Antibody ArrayConditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Growth Factor Antibody Array - Membrane (41 Targets) (ab134002)Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.
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Quantification of Human Growth Factor Antibody ArrayHuman serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
Protocols
References
This product has been referenced in:
- Shiga Y et al. Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period. Asian Spine J 11:329-336 (2017). Read more (PubMed: 28670400) »
- Sie M et al. Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma. PLoS One 10:e0122555 (2015). Human . Read more (PubMed: 25799134) »
