Overview

  • Product name

    Human Growth Hormone ELISA Kit
    See all Human Growth Hormone kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 3.6%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 2.4%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.6 pg/ml
  • Range

    9.4 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 94 91% - 100%
    Urine 112 107% - 119%
    Serum 103 97% - 109%
    Cell culture media 93 90% - 98%
    Heparin Plasma 97 94% - 99%
    EDTA Plasma 93 87% - 98%
    Citrate Plasma 97 92% - 102%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    As of March 1, 2019, Human Growth Hormone SimpleStep ELISA® kit has been re-developed with new recombinant monoclonal antibodies that provide a higher sensitivity when quantifying Growth Hormone protein in human serum, plasma, milk, urine, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulations hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    10X Human Growth Hormone Capture Antibody 1 x 600µl
    10X Human Growth Hormone Detector Antibody 1 x 600µl
    Human Growth Hormone Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similarities

    Belongs to the somatotropin/prolactin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • gH
    • GH-N
    • GH1
    • GHN
    • Growth hormone
    • Growth hormone 1
    • HGH
    • Pituitary growth hormone
    • SOMA_HUMAN
    • Somatotropin
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab190811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between human Growth Hormone SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Standard Curve comparison between human Growth Hormone new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.

  • The Growth Hormone standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: mixed gender serum 100% and pregnant serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 203.3 pg/mL in neat pooled mixed gender serum and 5,327 pg/mL in neat pooled pregnant female serum.

  • The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 309.0 pg/mL in neat plasma (citrate), 329.1 pg/mL in neat plasma (heparin), and 120 pg/mL in and neat plasma (EDTA).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

This product has been referenced in:

  • Kaklamanos A  et al. Extensive phenotypic characterization of a new transgenic mouse reveals pleiotropic perturbations in physiology due to mesenchymal hGH minigene expression. Sci Rep 7:2397 (2017). Read more (PubMed: 28546545) »
See 1 Publication for this product

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