• Product name
    Human Haptoglobin ELISA Kit
    See all Haptoglobin kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.9%
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type
    Serum, Plasma
  • Assay type
  • Sensitivity
    = 0.07 µg/ml
  • Range
    0.078 µg/ml - 20 µg/ml
  • Recovery

    = 98 %

  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Haptoglobin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.

    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    2X Biotinylated Human Haptoglobin (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 8ml
    Haptoglobin Microplate (12 x 8 well strips) 1 unit
    Haptoglobin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • Zonulin
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.


  • Haptoglobin measured in various biological fluids showing quantity (μg) per mL of sample tested

  • Representative Standard Curve using ab108856.



This product has been referenced in:
  • Perek B  et al. Late subclinical hemolysis and long-term outcomes after aortic valve replacement with On-X mechanical prostheses - a preliminary single-center report. Kardiochir Torakochirurgia Pol 14:175-179 (2017). Read more (PubMed: 29181045) »
  • Chang G  et al. Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver. BMC Vet Res 11:67 (2015). Read more (PubMed: 25889631) »

See all 2 Publications for this product

Customer reviews and Q&As

You do not need to subtract a blank for competitive ELISA. If you do, you will end up with negative values.

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit

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Thank you for your inquiry.

I heard back from the lab that serum that has undergone hemolysis will have a small interference with the haptoglobulin, but you should be able to use the sample. You should do a background control in this case. Read More

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.
The Haptoglobulin Human ELISA Kits (ab108856 and ab108858) can be used in order to quantify the level of haptoglobulin in various biolo...

Read More

Thank you very much for your interest in ab108856

To our knowledge, ab108856 has not been tested in Urine samples. However by participating in our AbTrial program you can now use our products in an untested application or species without fin...

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Thank you very much for your reply and for checking on the other 2 kits.

I apologize again for these errors. I looked through our records, and we haven't received any other reports of problems with ab108856, so I think that the quality of the...

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Thank you very much for your email and for letting us know about the trouble with this kit.

I've checked all of our stock notes on this kit, and I've examined 3 of the kits that we have in stock. The haptoglobin standard should have arrived l...

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Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have received the following information from the lab:

The proper value for Haptoglobulin in human pl...

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Te pido disculpas por la espera y agradezco tu paciencia.

Me confirman mis compañeros del laboratorio que ambos kits (ab108856 y ab108858) detectan la subunidad beta de la proteína. Además, si la sensibilidad del kit ab10...

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Thank you for your reply.

The standard curve you had sent me originally looked good. I assume these are the same data.

I apologize, I was mistaken.The blank has the highest value, thus you would not be able to subtract it from the ...

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1-10 of 16 Abreviews or Q&A


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