Overview

  • Product name

    Human Haptoglobin ELISA Kit
    See all Haptoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.6%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 2.8 ng/ml
  • Range

    3.125 ng/ml - 200 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Haptoglobin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin concentrations in Human urine, saliva, milk and cell culture supernatants.


    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human Haptoglobin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Haptoglobin Microplate (12 x 8 well strips) 1 unit
    Haptoglobin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • HPT_HUMAN
    • Zonulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108858.

Protocols

References

This product has been referenced in:

  • Wagener BM  et al. Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study. PLoS Med 15:e1002522 (2018). Read more (PubMed: 29522519) »
  • Oh JY  et al. Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices. Redox Biol 9:167-177 (2016). Read more (PubMed: 27566280) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us and sorry for the delay in getting back to you. Your customer is correct in thinking that the Haptoglobin and Haptoglobulin refer to the same protein. I am sorry that our datasheets have caused confusion. I will be looking into remedying this to use a uniform name for the products. I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for your interest in the haptoglobin sandwich ELISA, ab108858. It can be used to assay plasma, and the citrate will not interfere. You will want to test serial dilutions to find out which will be within the range of the standards, as it is expected that levels in plasma are much greater than in the fluids listed on the datasheet. The competitive ELISA ab108856 protocol recommends diluting plasma and serum samples 1/2000 before assaying, and as you will see in the protocol, the ab108856 standards are about 100X more concentrated (0.078 - 20 ug/mL) than the standards for ab108858 (3 - 200 ng/mL). So, you may need to dilute the plasma samples as much as 1/200,000 before assaying with ab108858.

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Answer

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.
The Haptoglobulin Human ELISA Kits (ab108856 and ab108858) can be used in order to quantify the level of haptoglobulin in various biological samples. With conditioned culture media samples the kit ab108858 would be most appropriate to use. This has been used with cell culture supernatant, saliva, milk, and urine.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

Read More

Answer

Te pido disculpas por la espera y agradezco tu paciencia.

Me confirman mis compañeros del laboratorio que ambos kits (ab108856 y ab108858) detectan la subunidad beta de la proteína. Además, si la sensibilidad del kit ab108856 os conviene más, no hay inconvenientes en usarlo en muestras de orina, pero en este tipo de muestra no lo hemos optimizado, por lo que tendréis que probar distintas condiciones para encontrar las más adecuadas a vuestro experimento concreto.

Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

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