Overview

  • Product name

    Human Haptoglobin Matched Antibody Pair Kit
    See all Haptoglobin kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    312.5 pg/ml - 20000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant Human Haptoglobin.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


     


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


     


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab219048) which uses the same antibody pair.


     


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human Haptoglobin Capture Antibody 1 x 100µg 1 x 20µg
    Human Haptoglobin Detector Antibody 1 x 25µg 1 x 5µg
    Human Haptoglobin Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Anhaptoglobinemia
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Binding peptide
    • BP
    • Haptoglobin alpha chain
    • Haptoglobin alpha(1S) beta
    • Haptoglobin alpha(2FS) beta
    • Haptoglobin beta chain
    • Haptoglobin, alpha polypeptide
    • Haptoglobin, beta polypeptide
    • HP
    • HP2 ALPHA2
    • HP2ALPHA2
    • HPA1S
    • HPT
    • HPT_HUMAN
    • MGC111141
    • Zonulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab219542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab219542 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219542.
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