• Product name
    Human HB EGF ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    16.38 pg/ml - 4000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 81.22 73% - 90%
    Plasma 93.73 84% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100531

  • Representative standard curve using ab100531



This product has been referenced in:
  • Wang X  et al. Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier. PLoS Pathog 12:e1005926 (2016). Read more (PubMed: 27711202) »

See 1 Publication for this product

Customer reviews and Q&As

1. Increase amount of biotinylated ab (by 1.5 fold or so – too much may increase background)
2. Incubate biotinylated ab overnight at 4 degrees C
3. Increase amount of HRP-strep (by about 1.5 fold or so – to...

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Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacell...

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Thank you for contacting us.

I am very sorry to hear these kits failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to ...

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Thank you for the updates.

We rarely recommend running plasma or serum samples undiluted, as matrix effects are more likely to occur. For this kit (ab100531: HB EGF Human ELISA Kit), we recommend a 2-fold dilution of plasma or serum. This may...

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Muchas gracias por contactarnos. Le pido disculpas por la espera.

El kit al que se refiere es valido para detectar HB EGF humano en suero, plasma y sobrenadante de cultivo celular. En el protocolo adjunto en la página web se indican lo...

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Thank you for the enquiry regarding ab100531 and for sending the customer’s data/comments.

Please find enclosed the attached file for the recent analysis. As you can see, the standard curves obtained have good linearity and very low CV%...

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Thank you for your enquiry regarding ab100531. I am very sorry to hear that your customer having problems with this kit.

The batch number you have provided (81802) does not match to our record. Could you please check it again and confirm the ...

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Thank you for your call yesterday and for your questions. I have been in touch with my colleague in the lab, and have received more information about ab100531.

The standard provided in ab100531 is expressed in baculovirus, and it corresonds t...

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Thank you for contacting Abcam.

Youshould use Assay Diluent C if diluting conditioned media samples.

Please letme knowif you haveany questions.

Thank you for contacting Abcam.

Please find the answers to your questions below:

1. are the coating antibody and the detection antibody a polyclonal or
monoclonal antibodies?Capture antibody = monoclonal
Detection antibody...

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