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    human-hbb-elisa-kit-ab235654.pdf

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RecombinantSimpleStep

Human HBB ELISA Kit (ab235654)

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  • SDS
  • Protocol Booklet
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Other - Human HBB ELISA Kit (ab235654)
  • Example of human HBB standard curve in Sample Diluent NS
  • Interpolated concentrations of native HBB in human serum and plasma samples
  • Interpolated concentrations of native HBB in TF-1 Extract based on a 37.5 µg/mL extract load
  • Sandwich ELISA - Human HBB ELISA Kit (ab235654)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 34.1 pg/ml
  • Range: 0.125 ng/ml - 8 ng/ml
  • Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human HBB ELISA Kit
    See all Hemoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.6%
  • Sample type

    Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    34.1 pg/ml
  • Range

    0.125 ng/ml - 8 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102 % - %
    Cell culture extracts 90 % - %
    Hep Plasma 101 % - %
    EDTA Plasma 107 % - %
    Cit plasma 98 % - %
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human HBB ELISA Kit (ab235654) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HBB protein in cell culture extracts, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HBB with 34.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human HBB Capture Antibody 1 x 600µl
    10X Human HBB Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human HBB Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits
  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity

    Red blood cells.
  • Involvement in disease

    Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • Sequence similarities

    Belongs to the globin family.
  • Post-translational
    modifications

    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Target information above from: UniProt accession P69905 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 3-prime alpha-globin gene
    • A gamma globin
    • Alpha 1 globin
    • Alpha 2 globin
    • Alpha 2 globin chain
    • Alpha globin
    • alpha one globin
    • alpha-1 globin
    • Alpha-globin
    • Beta globin
    • CD113t C
    • CD31
    • Delta globin
    • Erythremia, beta-globin type, included
    • Gamma 1 globin
    • Gamma A hemoglobin
    • Gamma globin
    • Hb F Agamma
    • HBA
    • HBA 1
    • HBA 2
    • HBA T3
    • HBA-T2
    • HBA_HUMAN
    • HBA1
    • HBA2
    • HBB
    • Hbb-y
    • HBD
    • Hbe1
    • HBG
    • HBG 1
    • HBG1
    • HBGA
    • HBGR
    • HBH
    • Hemoglobin alpha 1
    • Hemoglobin alpha 1 chain
    • hemoglobin alpha 1 globin chain
    • hemoglobin alpha 2
    • Hemoglobin alpha chain
    • Hemoglobin alpha locus
    • Hemoglobin alpha locus 1
    • hemoglobin alpha-1 chain
    • Hemoglobin beta
    • Hemoglobin beta chain
    • Hemoglobin beta chain complex
    • Hemoglobin beta locus
    • Hemoglobin gamma
    • Hemoglobin gamma 1 chain
    • Hemoglobin gamma A
    • Hemoglobin gamma A chain
    • Hemoglobin subunit alpha
    • Hemoglobin subunit beta
    • Hemoglobin subunit gamma 1
    • hemoglobin, gamma, regulator of
    • Hemoglobin--gamma locus, 136 alanaine
    • HSGGL1
    • LVV-hemorphin-7
    • Methemoglobinemia, beta-globin type, included
    • MGC126895
    • MGC126897
    • Minor alpha-globin locus
    • PRO2979
    see all
  • Database links

    • Entrez Gene: 3047 Human
    • Entrez Gene: 3039 Human
    • Entrez Gene: 3040 Human
    • Entrez Gene: 3043 Human
    • Omim: 141900 Human
    • Omim: 141800 Human
    • Omim: 142200 Human
    • SwissProt: P68871 Human
    • SwissProt: P69891 Human
    • SwissProt: P69905 Human
    • Unigene: 523443 Human
    • Unigene: 449630 Human
    • Unigene: 654744 Human
    see all

Associated products

  • Alternative Versions

    • Human HBB Antibody Pair - BSA and Azide free (ab244139)

Images

  • Other - Human HBB ELISA Kit (ab235654)
    Other - Human HBB ELISA Kit (ab235654)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human HBB standard curve in Sample Diluent NS
    Example of human HBB standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native HBB in human serum and plasma samples
    Interpolated concentrations of native HBB in human serum and plasma samples

    The concentrations of HBB were measured in duplicates, interpolated from the HBB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (heparin) 5%, plasma (EDTA) 25% and plasma (citrate) 3.13% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBB concentration was determined to be 64.9 ng/mL in serum, 28.6 ng/mL in plasma (heparin), 27.1 ng/mL in plasma (EDTA) and 192.2 ng/mL in plasma (citrate).

  • Interpolated concentrations of native HBB in TF-1 Extract based on a 37.5 µg/mL extract load
    Interpolated concentrations of native HBB in TF-1 Extract based on a 37.5 µg/mL extract load

    The concentrations of HBB were measured in duplicate and interpolated from the HBB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBB concentration was determined to be 4.5 ng/mL in TF-1 extract.

  • Sandwich ELISA - Human HBB ELISA Kit (ab235654)
    Sandwich ELISA - Human HBB ELISA Kit (ab235654)
    To learn more about the advantages of recombinant antibodies see here.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab235654? Please let us know so that we can cite the reference in this datasheet.

    ab235654 has not yet been referenced specifically in any publications.

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