Overview

  • Product name

    Human Heat Shock Protein 47 ELISA Kit (SERPINH1)
    See all Hsp 47 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 8 5.9%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 10.1%
  • Sample type

    Cell culture extracts, Adherent cells, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    50 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 95% - 97%
    Cell culture media 102 96% - 108%
    Heparin Plasma 92 90% - 93%
    Citrate Plasma 88 87% - 90%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 47 (SERPINH1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Heat Shock Protein 47 in human tissue and cell extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Heat Shock Protein 47 is a collagen specific molecular chaperone protein found in the endoplasmic reticulum. HSP47 assists in the triple-helical formation of collagen and is part of the collagen quality control system. Collagen accounts for approximately 30% of the total protein found in vertebrates and is the major component responsible in providing structural support for a number tissues. HSP47 can be found constitutively expressed in non-stressed cells associated with collagen expression. Increased HSP47 expression has been associated with increase collagen deposition in arteriosclerosis, myocardial infarction, hepatic fibrosis, renal fibrosis, and pulmonary fibrosis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab202407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Extracts with a starting concentration of: A431 (100 µg/mL), MCF7 (50 µg/mL), and HepG2 (50 µg/mL) were assayed with a 2-fold dilution scheme. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Extracts with a starting concentration of: human heart extract (500 µg/mL) and human liver extract (250 µg/mL) were assayed with a 2-fold dilution scheme. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab202407 has not yet been referenced specifically in any publications.

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