Overview

  • Product name

    Human Anti-Helicobacter pylori IgA ELISA Kit
    See all Helicobacter pylori IgA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Standard B 12 7.2%
    Standard C 16 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Standard B 3 9.6%
    Standard C 3 6.7%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Sensitivity

    < 5 U/ml
  • Range

    5 U/ml - 150 U/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Helicobacter pylori IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgA class antibodies against Helicobacter pylori in Human serum and plasma.

     

    A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgA sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108762

Protocols

References

ab108762 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

To the best of our knowledge, there is not cross reactivity with IgG.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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