Human Anti-Helicobacter pylori IgG ELISA Kit (ab108736)
Key features and details
- Sensitivity: 3 U/ml
- Range: 3 U/ml - 150 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Helicobacter pylori IgG ELISA Kit
See all Helicobacter pylori IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos.Serum 19 6.1% Pos.Serum 19 7% Inter-assay Sample n Mean SD CV% Pos.Serum 13 7.2% Pos.Serum 13 3% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
3 U/ml -
Range
3 U/ml - 150 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.
A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Helicobacter pylori (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Helicobacter pylori anti-IgG HRP conjugate Blue, Black cap 1 x 20ml Helicobacter pylori IgG Standard A – 0 U/mL Blue Cap 1 x 2ml Helicobacter pylori IgG Standard B – 15 U/mL Green Cap 1 x 2ml Helicobacter pylori IgG Standard C – 75 U/mL Yellow cap 1 x 2ml Helicobacter pylori IgG Standard D – 150 U/mL Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab108736 has been referenced in 5 publications.
- Zyambo K et al. Evaluation of the association between gastric cancer and plasma selenium in Zambian adults: a case-control study. Ecancermedicalscience 16:1351 (2022). PubMed: 35242232
- Mallah N et al. A multicenter case-control study of the effect of e-nos VNTR polymorphism on upper gastrointestinal hemorrhage in NSAID users. Sci Rep 11:19923 (2021). PubMed: 34620931
- Mallah N et al. Influence of Polymorphisms Involved in Platelet Activation and Inflammatory Response on Aspirin-Related Upper Gastrointestinal Bleeding: A Case-Control Study. Front Pharmacol 11:860 (2020). PubMed: 32655394
- Fulgione A et al. Interaction between MyD88, TIRAP and IL1RL1 against Helicobacter pylori infection. Sci Rep 10:15831 (2020). PubMed: 32985578
- Fulgione A et al. Epistatic interaction between MyD88 and TIRAP against Helicobacter pylori. FEBS Lett 590:2127-37 (2016). PubMed: 27296467