Product nameHuman Heme Oxygenase 1 Matched Antibody Pair Kit
See all Heme Oxygenase 1 kits
Assay typeELISA set
Range62.5 pg/ml - 4000 pg/ml
Species reactivityReacts with: Human
Human Heme Oxygenase 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Heme Oxygenase 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab207621. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.
Tested applicationsSuitable for: IA, ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 5 x 96 tests 2 x 96 tests Heme Oxygenase 1 Capture Antibody 1 x 100µg 1 x 50µg 1 x 20µg Heme Oxygenase 1 Detector Antibody 1 x 25µg 1 x 12.5µg 1 x 5µg Heme Oxygenase 1 Lyophilized Protein 1 vial 1 vial 1 vial
FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
Sequence similaritiesBelongs to the heme oxygenase family.
Cellular localizationMicrosome. Endoplasmic reticulum.
- Information by UniProt
- 32 kD
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab215401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ab215401 has not yet been referenced specifically in any publications.