Overview

  • Product name

    Human Hemoglobin subunit alpha Matched Antibody Pair Kit
    See all Hemoglobin subunit alpha kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    500 pg/ml - 32000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Hemoglobin subunit alpha.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab219049) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human Hemoglobin subunit alpha Capture Antibody 1 x 100µg 1 x 20µg
    Human Hemoglobin subunit alpha Detector Antibody 1 x 25µg 1 x 5µg
    Human Hemoglobin subunit alpha Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity

    Red blood cells.
  • Involvement in disease

    Heinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • Sequence similarities

    Belongs to the globin family.
  • Post-translational
    modifications

    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Alternative names

    • Alpha 1 globin
    • Alpha globin
    • Alpha one globin
    • Alpha-globin
    • HBA_HUMAN
    • HBA1
    • HBA2
    • Hemoglobin alpha 1
    • Hemoglobin alpha 1 chain
    • Hemoglobin alpha 1 globin chain
    • Hemoglobin alpha 2
    • Hemoglobin alpha chain
    • Hemoglobin subunit alpha
    • MGC126895
    • MGC126897
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab222265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222265.
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