Overview

  • Product name

    Human Hemopexin ELISA Kit (HPX)
    See all Hemopexin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type

    Milk, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    > 0.7 µg/ml
  • Range

    0.078 µg/ml - 5 µg/ml
  • Recovery

    98 %

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.


    Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample

  • Representative Standard Curve using ab108859.

Protocols

References

This product has been referenced in:

  • Lawson HA  et al. Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans. Int J Obes (Lond) 41:631-638 (2017). Read more (PubMed: 28119529) »
  • Evans C  et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). Read more (PubMed: 26315932) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abreviews
excellent products, and easy use to use

Abcam user community

Verified customer

Submitted Jan 24 2018

Hemopexin (HPX) Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: EDTA human plasma stored at -80ºC
Amount of Sample: 3 l
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 3 l of plasma in 1200 l of 1XDiluent N (1:400)
Washings were done on a Microplate Washer machine with 300 l of wash buffer.
The standards data generated an excellent curve.
All samples assayed showed data inside the curve.

Abcam user community

Verified customer

Submitted Apr 08 2015

Question
Answer

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2) Apolipoprotein E Human ELISA Kit
3) Haptoglobin Human ELISA Kit
4) Hemopexin Human ELISA Kit
5) Kininogen Human ELISA Kit
6) Retinol binding Protein 4 Human ELISA Kit

I'm sorry that this information could not be more helpful. Please contact us with any other questions.

Read More

Answer

Thank you for your enquiry.

Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Standard solution.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Abreview for Hemopexin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum
Amount of Sample: 2 ul µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
We washed the plate 6 times with a machine.
The standards data generated an excelent curve with a fitting error of
3.48e-5.
All samples assayed showed data inside the curve.

Dr. Rafael Montes Nieto

Verified customer

Submitted Mar 26 2012

Answer

Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.

Read More

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