Key features and details
- Sensitivity: 0.62 ng/ml
- Range: 0.25 µg/ml - 1 µg/ml
- Sample type: Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameHuman Hemopexin ELISA Kit (HPX)
See all Hemopexin kits
Intra-assay Sample n Mean SD CV% Overall 5.5% Inter-assay Sample n Mean SD CV% Overall 10.5%
Sample typeMilk, Serum, Plasma
Sensitivity> 0.62 ng/ml
Range0.25 µg/ml - 1 µg/ml
Assay time2h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.
Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Hemopexin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 8ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Tissue specificityExpressed by the liver and secreted in plasma.
Sequence similaritiesBelongs to the hemopexin family.
Contains 5 hemopexin-like domains.
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Information by UniProt
- Beta 1B glycoprotein
ab108859 has been referenced in 3 publications.
- Lawson HA et al. Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans. Int J Obes (Lond) 41:631-638 (2017). PubMed: 28119529
- Evans C et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). PubMed: 26315932
- Insenser M et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). PubMed: 24979072