Human Hemopexin ELISA Kit (HPX) (ab108860)

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Overview

  • Product name

    Human Hemopexin ELISA Kit (HPX)
    See all Hemopexin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.3%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 6 ng/ml

  • Range

    6.25 ng/ml - 400 ng/ml

  • Recovery

    98 %

  • Assay time

    3h 30m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.


    Get higher sensitivity in only 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Sandwich ELISA - Hemopexin (HPX) Human ELISA Kit (ab108860)
    Sandwich ELISA - Hemopexin (HPX) Human ELISA Kit (ab108860)

    Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108860.

Protocols

References

This product has been referenced in:

  • Zheng W  et al. Cerebrospinal Fluid Proteins Identification Facilitates the Differential Diagnosis of Central Nervous System Diffuse Large B Cell Lymphoma. J Cancer 8:3631-3640 (2017). Functional Studies . Read more (PubMed: 29151949) »
  • Zager RA  et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . Read more (PubMed: 22993068) »
See all 2 Publications for this product

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