Human Hemopexin ELISA Kit (HPX) (ab108860)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Hemopexin ELISA Kit (HPX)
See all Hemopexin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.3% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 6 ng/ml -
Range
6.25 ng/ml - 400 ng/ml -
Recovery
98 %
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Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.
Get higher sensitivity in only 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Hemopexin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
Images
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Sandwich ELISA - Hemopexin (HPX) Human ELISA Kit (ab108860)
Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.
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Typical Standard CurveRepresentative Standard Curve using ab108860.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108860 has been referenced in 2 publications.
- Zheng W et al. Cerebrospinal Fluid Proteins Identification Facilitates the Differential Diagnosis of Central Nervous System Diffuse Large B Cell Lymphoma. J Cancer 8:3631-3640 (2017). Functional Studies . PubMed: 29151949
- Zager RA et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . PubMed: 22993068