Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab31875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Hepcidin-25 antibody (ab30760)

    ELISA

  • Purity
    70 - 90% by HPLC.

  • Form
    Liquid
  • Additional notes

    Storage buffer: 10% 0.5M acetic acid, 90% TK buffer. pH 3.6

    Solutions of ab31875, diluted 10 times, in TBST (80%) and PBS (10%) have a pH of 5.1

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Hamp
    • HEPC
    • HEPC_HUMAN
    • Hepc20
    • Hepc25
    • HEPCIDIN
    • Hepcidin 20
    • Hepcidin 25
    • Hepcidin antimicrobial peptide
    • Hepcidin-20
    • Hepcidin25
    • HFE2
    • HFE2B
    • LEAP 1
    • LEAP-1
    • LEAP1
    • Liver expressed antimicrobial peptide
    • Liver-expressed antimicrobial peptide 1
    • PLTR
    • Putative liver tumor regressor
    see all
  • Function
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • Tissue specificity
    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • Involvement in disease
    Hemochromatosis 2B
  • Sequence similarities
    Belongs to the hepcidin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

This product has been referenced in:
  • Xiong XY  et al. Toll-Like Receptor 4/MyD88-Mediated Signaling of Hepcidin Expression Causing Brain Iron Accumulation, Oxidative Injury, and Cognitive Impairment After Intracerebral Hemorrhage. Circulation 134:1025-1038 (2016). Read more (PubMed: 27576776) »
  • McCarthy RC & Kosman DJ Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS One 9:e89003 (2014). Read more (PubMed: 24533165) »
See all 2 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
ELISA
Review text: Used in conjunction with ab30760 at does dependent concentration as a positive control.
Sample: Mouse Recombinant protein (Mouse Primary neurons)
Specification: Mouse Primary neurons
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type: Direct

Other product details
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: ([2.93g Sodium Bicarbonate (NaHCO3), 1.5

Primary antibody (in addition to 'Hepcidin-25 peptide')
Dilution: 1/5000

Secondary antibody
Name: Non-Abcam antibody was used: Dako goat anti rabbit HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/50000

Abcam user community

Verified customer

Submitted Oct 09 2012

Answer

Thank you for contacting Abcam regarding ab31875.


The buffer contains the following:


30 mM HEPES
150 mM NaCl
2 mM EDTA
0.001% Tween 20
At pH 6.75


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Merci pour la confirmation.

Comme convenu, j'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est *******.

Voici les manières dont vous pouvez utiliser cette référence CN:
(1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée
(2) la faire valoir contre une prochaine facture
(3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam

Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse mailto:creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité.

Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB.

J'espère que cette expérience ne vous empêchera pas de commander à nouveau l'un de nos produits.

L'équipe technique reste à votre disposition pour plus de conseils.

Bonne continuation dans votre recherche.

Read More

Answer

Merci de nous avoir contactés.

Je suis désolé que le produit ne fonctionne pas.

L'explication est très probablement la suivante: la longueur de ce peptide n'est que de 25 acides aminés (aa 60-84 de l'hépicine humaine). Ce peptide sert d'immunogène de l'anticorps ab75883.

J'ai parcouru la fiche technique qui, j'en suis désolé, contient une information qui porte à confusion , à savoir la longueur de la protéine (et non du peptide) qui est de 84 acides aminés.

J'ai demandé à ce que cette information soit reformulée ou supprimée de la fiche technique.

Nous n'avons pas à notre catalogue l'hépicine entière. Je veux bien néanmoins procéder à l'édition d'un avoir / remboursement en compensation de cette confusion.

Merci de me confirmer que cette résolution vous convient.

Read More

Answer

Thank you for contacting us.

The peptide should already be in solution. Is that not the case? You should have 100ul. Be sure to centrifuge the tube to pulll down any solution trapped in the threads of the tube.

If you want to further dilute the stock solution, distilled water will be fine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

The product ab31875 is a synthetic 25 amino acid peptide that is not, to our knowledge, active. This peptide has been used to produce the antibody ab75883, which is validated for use on mouse and human samples in WB, IHC-Fr, and ICC/IF. I hope this helps, please let me know if you have any other questions.

Read More

Answer

Thank you for your reply.

I am still concerned that the resolving conditions are not suitable for such a small protein. I will send on the peptide as an exception. However, I am not sure how it will be used for blocking if a band of the appropriate size is not detected.

Please confirm the order number and date of shipping so that I can send on the peptide. Looking forward to your reply.

Read More

Answer

Thanks you for your email. Dr. Tanya is away this week so I am looking after her inbox. The peptide sequence for ab31876 corresponds to amino acid XXXX and peptide sequence for ab31875 corresponds to XXXX amino acids. The antibody cross reactivity always depends on the immunogen sequences, so customers will automatically know from the datasheet e.g. which part of the immunogen the antibody is specific to and in which part of the processed protein, the immunogen lies. This is very obvious information so we do not think we need to publish this information. However we are always happy to answer any inquiry we receive regarding there products. I hope this will be helpful. Should you any other please does not hesitate to contact us.

Read More

Answer

Thank you for your enquiry and your interest in our products. I have conducted a quick blast and the peptide sequence picks up both hepcidin and hepcidin 25. The peptide sequence corresponds to aas 60-84 of the full length protein. The antibody recognizes the processed hepcidin 25 (60-84 aa) which will be found at low concentrations in the blood (presumably) and it will also detect the full length pre-pro protein which would be found in the cell – hence easier to run on a Western blot. Therefore it would be very difficult to get an antibody specific for the processed protein. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting us. This peptide was synthesised on solid phase, using Fmoc-protected amino acids. Amino acid side-chains are protected. The full length peptide is cleaved from the resin and fully deprotected using 95% TFA and scavenger mix to remove protecting group by-products. The crude peptide is purified by reverse phase HPLC, using aqueous acetonitrile with 0.1% gradient. The peptide pool is then lyophilized, and is supplied in fully reduced form (ie no disulfide bridges), and TFA salt. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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