Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab31875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Hepcidin-25 antibody (ab30760)

    ELISA

  • Purity
    70 - 90% by HPLC.

  • Form
    Liquid
  • Additional notes

    Storage buffer: 10% 0.5M acetic acid, 90% TK buffer. pH 3.6

    Solutions of ab31875, diluted 10 times, in TBST (80%) and PBS (10%) have a pH of 5.1

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Hamp
    • HEPC
    • HEPC_HUMAN
    • Hepc20
    • Hepc25
    • HEPCIDIN
    • Hepcidin 20
    • Hepcidin 25
    • Hepcidin antimicrobial peptide
    • Hepcidin-20
    • Hepcidin25
    • HFE2
    • HFE2B
    • LEAP 1
    • LEAP-1
    • LEAP1
    • Liver expressed antimicrobial peptide
    • Liver-expressed antimicrobial peptide 1
    • PLTR
    • Putative liver tumor regressor
    see all
  • Function
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • Tissue specificity
    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • Involvement in disease
    Hemochromatosis 2B
  • Sequence similarities
    Belongs to the hepcidin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

This product has been referenced in:
  • Xiong XY  et al. Toll-Like Receptor 4/MyD88-Mediated Signaling of Hepcidin Expression Causing Brain Iron Accumulation, Oxidative Injury, and Cognitive Impairment After Intracerebral Hemorrhage. Circulation 134:1025-1038 (2016). Read more (PubMed: 27576776) »
  • McCarthy RC & Kosman DJ Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS One 9:e89003 (2014). Read more (PubMed: 24533165) »
See all 2 Publications for this product

Customer reviews and Q&As

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Application
ELISA
Review text: Used in conjunction with ab30760 at does dependent concentration as a positive control.
Sample: Mouse Recombinant protein (Mouse Primary neurons)
Specification: Mouse Primary neurons
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type: Direct

Other product details
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: ([2.93g Sodium Bicarbonate (NaHCO3), 1.5

Primary antibody (in addition to 'Hepcidin-25 peptide')
Dilution: 1/5000

Secondary antibody
Name: Non-Abcam antibody was used: Dako goat anti rabbit HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/50000

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Verified customer

Submitted Oct 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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