Human Hepcidin-25 peptide (ab31876)

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Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
      P81172
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab31876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Hamp
    • HEPC
    • HEPC_HUMAN
    • Hepc20
    • Hepc25
    • HEPCIDIN
    • Hepcidin 20
    • Hepcidin 25
    • Hepcidin antimicrobial peptide
    • Hepcidin-20
    • Hepcidin25
    • HFE2
    • HFE2B
    • LEAP 1
    • LEAP-1
    • LEAP1
    • Liver expressed antimicrobial peptide
    • Liver-expressed antimicrobial peptide 1
    • PLTR
    • Putative liver tumor regressor
    see all
  • Function
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • Tissue specificity
    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • Involvement in disease
    Hemochromatosis 2B
  • Sequence similarities
    Belongs to the hepcidin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab31876 has not yet been referenced specifically in any publications.

Publishing research using ab31876? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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What is the hepcidin-25 peptide delivered in?

The Hepcidin-25 peptide storage buffer for the current batch (GR125325-1) is as follows:

30 mM HEPES
150 mM NaCl
2 mM EDTA
0.001% Tween20
pH 6.75


Inquiry: Hello, we are looking for an endotoxin-free active form of Hepcidin to treat cells. Is this one inactive?

Thank you for contacting us. Yes, this is a smaller peptide from the Hepcidi-25 protein and is inactive. Please let me know if you have any further questions.

Thanks very much for your detailed response, that’s helpful. Just a couple of further questions/comments: when you state that the peptide sequence corresponds to aas 60-84, are you referring to ab31876 or ab31875 or both? There is evidence in th...

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Thanks you for your email. Dr. Tanya is away this week so I am looking after her inbox. The peptide sequence for ab31876 corresponds to amino acid XXXX and peptide sequence for ab31875 corresponds to XXXX amino acids. The antibody cross ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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