Human Hepcidin-25 peptide (ab31876)

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Description

Specifications

Our Abpromise guarantee covers the use of ab31876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form

    Liquid

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • Hamp
    • HEPC
    • HEPC_HUMAN
    • Hepc20
    • Hepc25
    • HEPCIDIN
    • Hepcidin 20
    • Hepcidin 25
    • Hepcidin antimicrobial peptide
    • Hepcidin-20
    • Hepcidin25
    • HFE2
    • HFE2B
    • LEAP 1
    • LEAP-1
    • LEAP1
    • Liver expressed antimicrobial peptide
    • Liver-expressed antimicrobial peptide 1
    • PLTR
    • Putative liver tumor regressor
    see all

  • Function

    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).

  • Tissue specificity

    Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.

  • Involvement in disease

    Hemochromatosis 2B

  • Sequence similarities

    Belongs to the hepcidin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab31876 has not yet been referenced specifically in any publications.

Publishing research using ab31876? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

What is the hepcidin-25 peptide delivered in?

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Answer

The Hepcidin-25 peptide storage buffer for the current batch (GR125325-1) is as follows:

30 mM HEPES
150 mM NaCl
2 mM EDTA
0.001% Tween20
pH 6.75

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Question


Inquiry: Hello, we are looking for an endotoxin-free active form of Hepcidin to treat cells. Is this one inactive?

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Answer

Thank you for contacting us. Yes, this is a smaller peptide from the Hepcidi-25 protein and is inactive. Please let me know if you have any further questions.

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Question

Thanks very much for your detailed response, that’s helpful. Just a couple of further questions/comments: when you state that the peptide sequence corresponds to aas 60-84, are you referring to ab31876 or ab31875 or both? There is evidence in the literature that pro-hepcidin is found in the blood as well as located intracellularly, and pre-pro-hepcidin can also be secreted from cells. I do think it might be helpful for customers to know that the ‘hepcidin-25’ antibodies are likely to recognise other forms of hepcidin (since it can be important to distinguish between active hepcidin and inactive precursors), and therefore it would be good to provide this information on the data sheet.  I hope to place an order for one of the hepcidin peptides and some anti-hepcidin antibodies soon. Best wishes,

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Answer

Thanks you for your email. Dr. Tanya is away this week so I am looking after her inbox. The peptide sequence for ab31876 corresponds to amino acid XXXX and peptide sequence for ab31875 corresponds to XXXX amino acids. The antibody cross reactivity always depends on the immunogen sequences, so customers will automatically know from the datasheet e.g. which part of the immunogen the antibody is specific to and in which part of the processed protein, the immunogen lies. This is very obvious information so we do not think we need to publish this information. However we are always happy to answer any inquiry we receive regarding there products. I hope this will be helpful. Should you any other please does not hesitate to contact us.

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