Product nameHuman Anti-Herpes simplex virus Type 1 and 2 IgM ELISA Kit (HSV1+2)
Intra-assay Sample n Mean SD CV% Pos.Serum 13 8.8% Inter-assay Sample n Mean SD CV% Pos.Serum 4 11.4%
Sample typeSerum, Heparin Plasma, Citrate Plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Herpes simplex virus Type 1 and 2 (HSV1+2) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Herpes simplex virus Type 1 and 2 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 1 and 2 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 1 and 2-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 1 and 2 IgM sample captured in plate.
Tested applicationsSuitable for: Indirect ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Herpes simplex virus Type 1+2 (IgM) Coated Microplate (12 x 8 wells) 1 unit Herpes simplex virus Type 1+2 anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Herpes simplex virus Type 1+2 IgM Cut-off Control Yellow, Green cap 1 x 3ml Herpes simplex virus Type 1+2 IgM Negative Control Yellow, Blue cap 1 x 2ml Herpes simplex virus Type 1+2 IgM Positive Control Yellow, Red cap 1 x 2ml IgM Sample Diluent Green, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Herpes Simplex Virus 1 (HSV 1) IgM
- Herpes Simplex Virus 1 IgM
- Herpes Simplex Virus 2 (HSV 2) IgM
Our Abpromise guarantee covers the use of ab108742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108742 has not yet been referenced specifically in any publications.