Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Herpes simplex virus Type 1 IgG ELISA Kit (HSV1)
Intra-assay Sample n Mean SD CV% Pos.Serum 20 3.4% Pos.Serum 24 3.7% Inter-assay Sample n Mean SD CV% Pos.Serum 12 2.7% Pos.Serum 12 4.9%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Herpes simplex virus Type 1 (HSV1) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Herpes simplex virus Type 1 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 1 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 1-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 1 IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Herpes simplex virus Type 1 (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Herpes simplex virus Type 1 anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Herpes simplex virus Type 1 IgG Cut-off Control Yellow, Green cap 1 x 3ml Herpes simplex virus Type 1 IgG Negative Control Yellow, Blue cap 1 x 2ml Herpes simplex virus Type 1 IgG Positive Control Yellow, Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108737 has been referenced in 1 publication.
- Salovin A et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). PubMed: 29629396