Overview

  • Product name

    Human Anti-Herpes simplex virus Type 1 IgG ELISA Kit (HSV1)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 20 3.4%
    Pos.Serum 24 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 12 2.7%
    Pos.Serum 12 4.9%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Herpes simplex virus Type 1 (HSV1) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Herpes simplex virus Type 1 in Human serum and plasma.

    A 96-well plate has been precoated with Herpes simplex virus Type 1 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 1-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 1 IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References

This product has been referenced in:

  • Salovin A  et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). Read more (PubMed: 29629396) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your email.

The product I have identified for you are as follows;

ab108776
ab108778

ab108767
we do not have anti rubella IgM kit

ab108724
ab108725

ab108737
ab108738

ab108739
ab108740

ab108742

I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

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Answer

Thank you very much for your inquiry.

I am happy to confirm that we do offer the following ELISA kits to analyse human Herpes Simplex Virus 1 and 2 that use the glycoprotein (1 or 2respectively)as antigens:

ab108737

https://www.abcam.com/index.html?datasheet=108737 (or use the following: https://www.abcam.com/index.html?datasheet=108737).

ab108738

https://www.abcam.com/index.html?datasheet=108738 (or use the following: https://www.abcam.com/index.html?datasheet=108738).

ab108739

https://www.abcam.com/index.html?datasheet=108739 (or use the following: https://www.abcam.com/index.html?datasheet=108739).

ab108740

https://www.abcam.com/index.html?datasheet=108740 (or use the following: https://www.abcam.com/index.html?datasheet=108740).

I hope this information is helpful for your customer and wish you a good start to the new week.

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Answer

Thank you for contacting us.

The lab sent the following advice:

"The HSV-1 IgG ELISA Kit is a qualitative immunoassay to determine the presence or absence of IgG to HSV-1. The end result is not quantitative, however, the relative result is indicative of higher or lower titer.
The researcher can compare the results of different test runs to infer relative titers. The researcher should be catious, however, that they are not measuring run to run variability or even a slight decline in the readings as the kit ages.
Assuming the researcher is testing samples taken from different time points, it may be best to test the samples side by side within the same run as they are collected for relative comparison of antibody titer."

It might be advisable - if that is possible in your experimental set up - to collect all the samples and then run the assay for all samples together at the end (e.g. after 6 months) to be able to compare them.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your patience.

I have obtained the following additional information:

The kit can be used for semi-quantification. This means that the result in OD can be expressed in NTU (arbitrary units). Therefore the OD of the sample is divided by the OD of the cut-off. This result is multiplied by 10. The result in NTU can be compared between different samples and different test runs. It is described in section 9 of the protocol
(https://www.abcam.com/Herpes-Simplex-Virus-1-HSV-1-IgG-Human-ELISA-Kit-ab108737-protocols.html or https://www.abcam.com/ps/products/108/ab108737/documents/ab108737%20Herpes%20Simplex%20Virus%201%20%20HSV%201%20%20IgG%20Human%20ELISA%20(Website).pdf).

It is not recommended to use the positive control for a standard curve since the linearity is not determined.

In general the concentration of the positive control is not determined in detail. For a qualitative test it is sufficient that the positive control is higher than the cut-off.



How many kits would you be interested in purchasing?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

I received the following information from the lab:

The HSV-1 IgG ELISA kit (ab108737) is intended for the qualitative determination of IgG to HSV-1. The kit is not designed to provide a quantitative result.

The lab is looking intothe concentration of the positive control and will let me know.

I have also asked them ifsemi-quantative results can be obtainedor whether only a Yes or No answer can be obtained. I will let you know as well, what Ifind out.


I hope this information is of some help to you so far. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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