Human Anti-Herpes simplex virus Type 2 IgG ELISA Kit (HSV2) (ab108739)

Submit a review Q&A (3)References (2)
Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    Overview

    • Product name

      Human Anti-Herpes simplex virus Type 2 IgG ELISA Kit (HSV2)

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Pos.Serum 20 3.4%
      Pos.Serum 24 3.7%
      Inter-assay
      Sample n Mean SD CV%
      Pos.Serum 12 3.6%
      Pos.Serum 12 5.1%

    • Sample type

      Serum, Hep Plasma, Cit plasma

    • Assay type

      Indirect

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s anti-Herpes simplex virus Type 2 (HSV2) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Herpes simplex virus Type 2 in Human serum and plasma.

      A 96-well plate has been precoated with Herpes simplex virus Type 2 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 2-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 2 IgG sample captured in plate.

    • Platform

      Microplate

    Properties

    Images

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    References (2)

    Publishing research using ab108739? Please let us know so that we can cite the reference in this datasheet.

    ab108739 has been referenced in 2 publications.

    • Salovin A  et al. Anti-NMDA receptor encephalitis and nonencephalitic HSV-1 infection. Neurol Neuroimmunol Neuroinflamm 5:e458 (2018). PubMed: 29629396
    • Slawinski BL  et al. Maternal cytomegalovirus sero-positivity and autism symptoms in children. Am J Reprod Immunol 79:e12840 (2018). PubMed: 29520885

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    We would be interested in obtaining a quote for the ToRCH range of assays. In particular:


    Toxoplasma IgG

    Toxoplasma IgM

    Rubella IgG

    Rubella IgM

    CMV IgG

    CMV IgM

    HSV-1 IgG

    HSV-2 IgG

    HSV-1 & 2 IgM


    We look forward to hearing from you soon.

    Kind regards,

    Read More
    Answer

    Thank you for your email.

    The product I have identified for you are as follows;

    ab108776
    ab108778

    ab108767
    we do not have anti rubella IgM kit

    ab108724
    ab108725

    ab108737
    ab108738

    ab108739
    ab108740

    ab108742

    I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

    I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

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    Question


    Inquiry: Dear Abcam, I am interested in purchasing your HSV-2 human IgG ELISA kit but I have the following questions: 1. What are the positive and negative controls? Ideally, they would be human serum samples with confirmed presence/absence of HSV-2 IgG, respectively. 2. The HSV-1/2 IgM kit also detects VZV. Does the HSV-2 IgG kit suffer from the the same limitation? 3. We wish to test ˜1200 patient samples. Would you offer a discount on a bulk order? Thanks for your help, Will

    Read More
    Answer

    Thank you for your message and inquiry regarding the HSV-2 IgG ELISA. The kit controls contain human serum or plasma samples. There is no cross reactivity with VZV IgG positive samples. We do offer a discount on bulk orders of 10 units or more. For more information on this please contact mailto:sales@abcam.com. Please let me know if you have any further questions.

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    Question

    One of our customer is looking for the following products with the mentioned specifications. Please let us know if ABCAM has these products with required specifications:



    Varicella Zoster 1g G Glycoprotein based Latex agglutinationor ELISA kit

    HERPES SIMPLES 1 & 2 KIT ELISA GLYCOPROTEIN BASE.



    Please let us have your feed back at an early time so that we may contact the institution accordingly.

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    Answer

    Thank you very much for your inquiry.

    I am happy to confirm that we do offer the following ELISA kits to analyse human Herpes Simplex Virus 1 and 2 that use the glycoprotein (1 or 2respectively)as antigens:

    ab108737

    https://www.abcam.com/Human-Herpes-simplex-virus-Type-1-IgG-ELISA-Kit-HSV1-ab108737.html (or use the following: https://www.abcam.com/Human-Herpes-simplex-virus-Type-1-IgG-ELISA-Kit-HSV1-ab108737.html).

    ab108738

    https://www.abcam.com/Human-Herpes-simplex-virus-Type-1-IgM-ELISA-Kit-HSV1-ab108738.html (or use the following: https://www.abcam.com/Human-Herpes-simplex-virus-Type-1-IgM-ELISA-Kit-HSV1-ab108738.html).

    ab108739

    https://www.abcam.com/Human-Herpes-simplex-virus-Type-2-IgG-ELISA-Kit-HSV2-ab108739.html (or use the following: https://www.abcam.com/Human-Herpes-simplex-virus-Type-2-IgG-ELISA-Kit-HSV2-ab108739.html).

    ab108740

    https://www.abcam.com/Human-Herpes-simplex-virus-Type-2-IgM-ELISA-Kit-HSV2-ab108740.html (or use the following: https://www.abcam.com/Human-Herpes-simplex-virus-Type-2-IgM-ELISA-Kit-HSV2-ab108740.html).

    I hope this information is helpful for your customer and wish you a good start to the new week.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com