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Signal Transduction Metabolism Energy Metabolism
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Recombinant

Human Hexokinase II Matched Antibody Pair Kit (ab220138)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human Hexokinase II standard curve.
  • Sandwich ELISA - Human Hexokinase II Matched Antibody Pair Kit (ab220138)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 31.3 pg/ml - 2000 pg/ml

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Protein
Product image
Recombinant human Hexokinase II protein (ab85920)

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Overview

  • Product name

    Human Hexokinase II Matched Antibody Pair Kit
    See all Hexokinase II kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    4.2 pg/ml
  • Range

    31.3 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Humn Hexokinase II Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Hexokinase II.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219043. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human Hexokinase II Capture Antibody 2 x 50µg 1 x 50µg
    Human Hexokinase II Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human Hexokinase II Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Tissue specificity

    Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • Pathway

    Carbohydrate metabolism; hexose metabolism.
  • Sequence similarities

    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • Domain

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localization

    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Target information above from: UniProt accession P52789 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • DKFZp686M1669
    • Hexokinase 2
    • Hexokinase 2 muscle
    • Hexokinase type II
    • Hexokinase-2
    • HK 2
    • HK II
    • HK2
    • HKII
    • HxK 2
    • HxK2
    • HXK2_HUMAN
    • Muscle form hexokinase
    see all
  • Database links

    • Entrez Gene: 3099 Human
    • Omim: 601125 Human
    • SwissProt: P52789 Human
    • Unigene: 406266 Human
    • Unigene: 591588 Human

    Associated products

    • Alternative Versions

      • Human Hexokinase II Antibody Pair - BSA and Azide free (ab241919)
    • Related Products

      • ELISA Accessory Pack (ab210905)
    • SimpleStep ELISA kits

      • Human Hexokinase II ELISA Kit (ab219043)

    Applications

    Our Abpromise guarantee covers the use of ab220138 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.

    Images

    • Human Hexokinase II standard curve.
      Human Hexokinase II standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human Hexokinase II Matched Antibody Pair Kit (ab220138)
      Sandwich ELISA - Human Hexokinase II Matched Antibody Pair Kit (ab220138)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab220138? Please let us know so that we can cite the reference in this datasheet.

    ab220138 has not yet been referenced specifically in any publications.

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