Human Hexokinase II Matched Antibody Pair Kit (ab220138)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Hexokinase II Matched Antibody Pair Kit
See all Hexokinase II kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
4.2 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Humn Hexokinase II Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Hexokinase II.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219043. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Hexokinase II Capture Antibody 1 x 100µg 1 x 50µg Human Hexokinase II Detector Antibody 1 x 25µg 1 x 12.5µg Human Hexokinase II Lyophilized Protein 1 vial 1 vial -
Research areas
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Tissue specificity
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. -
Pathway
Carbohydrate metabolism; hexose metabolism. -
Sequence similarities
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
Domain
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
Cellular localization
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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Alternative names
- DKFZp686M1669
- Hexokinase 2
- Hexokinase 2 muscle
see all -
Database links
- Entrez Gene: 3099 Human
- Omim: 601125 Human
- SwissProt: P52789 Human
- Unigene: 406266 Human
- Unigene: 591588 Human
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab220138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Human Hexokinase II standard curve.Standard calibration curve. Background subtracted values are graphed.
Protocols
References
ab220138 has not yet been referenced specifically in any publications.
