• Product name
    Human HGF ELISA Kit
    See all HGF kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.35 89% - 108%
    Serum 108.28 96% - 120%
    Plasma 107.56 95% - 119%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s HGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in serum, plasma (EDTA and citrate), and cell culture supernatants.

    This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • HuHGF measured in biological fluids showing quantity (pg) per mL of tested sample.

  • Representative standard curve using ab100534

  • Representative standard curve using ab100534



This product has been referenced in:
  • Wang X  et al. Cancer-associated fibroblasts induce epithelial-mesenchymal transition through secreted cytokines in endometrial cancer cells. Oncol Lett 15:5694-5702 (2018). Read more (PubMed: 29563996) »
  • Nishida S  et al. Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. Cancer Sci 104:431-6 (2013). Human . Read more (PubMed: 23320511) »

See all 2 Publications for this product

Customer reviews and Q&As

HGF ELISA in cell culture media

Good Good 4/5 (Ease of Use)
HGF ELISA worked well with cell cultured media.
I used the the kit as instruction provided in kit and cultured cell line media was used as it is without diluting.

Abcam user community

Verified customer

Submitted Apr 21 2017

Thank you for your inquiry. I have answered your questions below:

Does this kit detect total HGF - the single chain precursor of HGF (scHGF) and the activated heterodimer as well?

The exact form of HGF recognized has not been tested, ...

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I am sorry for the delay in getting back to you. I now have further data to share with you from the HGF Human ELISA Kit (ab100534).

Please find attached an excel spreadsheet. In the first sheet there is the data collected from two subsequent a...

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I am sorry for the delay in getting back to you. I now have further information to share with you in relation the variation seem by your customer when using the HGF Human ELISA Kit (ab100534).

The source of this kti ahve had a look at the resu...

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Thank you for contacting us.

The reconstituted standard is stable for ˜1 week if stored frozen (-20°C - -80°C). Please ensure avoiding multiple freeze – thaw cycles. We do provide 2 vials of the standard recombinant protein to m...

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Thank you for confirming that. xxxx is currently not in the office but I can help with this case.

I have now added the replacement kit to the order number xxxxxx. Please be aware that the kit may be moved from this order before shipping to be...

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Thank you for your reply.

It can't be that the buffer has the same consistency as glycerol!As we do not have separatecomponents, I would like to offer your customer a FOCR instead.

Please let me know if this is acceptable.

Thank you for sending in the data. The standard curve does still contain strong linearity when including the higher concentrated standards, 222.2 pg/ml and 666.7 pg/ml. The highest standard (2000 pg/ml) did give a reading that affects the linearity dow...

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Thank you for contacting us.

260 uL of Assay Diluent A or 1x Assay Diluent B should be added to all of the vials except the first one. The first vial should contain 390 uL of the Standard that was prepared in 600 uL of Assay Diluent A or B. T...

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It is difficult to recommend a specific speed for a "gentle shaking". The plate should just bemoving to get a homogenous solution and not too fast to avoid a spillage.I hope this helps.

1-10 of 15 Abreviews or Q&A


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