- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman HGF ELISA Kit
See all HGF kits -
Detection methodColorimetric
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Sample typeCell culture extracts, Tissue Extracts
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Assay typeSandwich (quantitative)
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Sensitivity< 8 pg/ml
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Range8.23 pg/ml - 6000 pg/ml
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Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture extracts 95 81% - 104% Tissue Extracts 95.5 80% - 103% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in cell lysates and tissue lysates.
This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysate Buffer 1 x 5ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Human HGF 2 vials HGF Microplate (12 x 8 wells) 1 unit Recombinant Human HGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
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Involvement in diseaseDeafness, autosomal recessive, 39
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Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
Database links
- Entrez Gene: 3082 Human
- Omim: 142409 Human
- SwissProt: P14210 Human
- Unigene: 396530 Human
- Unigene: 561679 Human
Applications
Our Abpromise guarantee covers the use of ab100535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical standard curveRepresentative standard curve using ab100535
Protocols
References
ab100535 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"