Human HGF ELISA Kit (ab100535)

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Typical standard curve

    Key features and details

    • Sensitivity: 8 pg/ml
    • Range: 8.23 pg/ml - 6000 pg/ml
    • Sample type: Cell culture extracts, Tissue Extracts
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human HGF ELISA Kit
      See all HGF kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture extracts, Tissue Extracts

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 8 pg/ml

    • Range

      8.23 pg/ml - 6000 pg/ml

    • Recovery

      95 %

      Sample specific recovery
      Sample type Average % Range
      Cell culture extracts 95 81% - 104%
      Tissue Extracts 95.5 80% - 103%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam is committed to meeting high quality standards of ethical manufacturing and will discontinue this product when inventory is depleted because it contains antibodies generated by the ascites method. We are sorry for the inconvenience this may cause.


      Abcam’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in cell lysates and tissue lysates.


      This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Notes

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Microplate

    Properties

    Associated products

      Images

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100535

      References (0)

      Publishing research using ab100535? Please let us know so that we can cite the reference in this datasheet.

      ab100535 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      Inquiry: I would like to know of this product if it recognizes the single chain and/or heterodimer variant of hepatocyte growth factor. Kind regards,

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      Answer


      This kit is expected to detect both the single unprocessed chain and the heterodimer HGF protein.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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