Human HGF ELISA Kit (ab100535)

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Overview

  • Product name

    Human HGF ELISA Kit
    See all HGF kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 8 pg/ml

  • Range

    8.23 pg/ml - 6000 pg/ml

  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 81% - 104%
    Tissue Extracts 95.5 80% - 103%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100535

Protocols

References

ab100535 has not yet been referenced specifically in any publications.

Publishing research using ab100535? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Inquiry: I would like to know of this product if it recognizes the single chain and/or heterodimer variant of hepatocyte growth factor. Kind regards,

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Answer


This kit is expected to detect both the single unprocessed chain and the heterodimer HGF protein.

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