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What is the excitation and emission wavelengths to be used in the Odyssey system with this kit?
Asked on Mar 07 2012
The IRDye labeled secondary antibodies provided in this kit are a mixture of IRDye 800 and IRDye 680 (standard Odyssey IR dyes that are read on the Odyssey scanner in the 800 and 700 channels). Section 8.11 in the protocol booklet. From LICOR’s website the Ex/Em spectra are 778/794 and 680/694 for IRDye 800 and IRDye 680, respectively.
We are not aware of a way to measure these dyes other than the Odyssey system.
I hope this is helpful. Please contact me again if you have any further questions.
Answered on Mar 07 2012