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I do not have a LI-COR-Odyssey or Aerius system. Can I use this kit with a standard microplate reader with OD595 as stated in the protocol (section 8.15)?
Asked on Sep 17 2014
Thank you for your inquiry. Unfortunately, you must have a LI-COR-Odyssey or Aerius system to use this kit. Measurement of Hif1a and PDK1 is done using IR dye conjugated secondary antibodies which need to be quantified using these systems.
Section 8.15 of the protocol refers to the optional normalization step which can be done using the Janus green dye. The Janus green dye can be read with the standard microplate.
I hope this helps!
Jared KaminskiAbcam Scientific Support
Answered on Sep 17 2014