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  1. Link

    human-histone-h12-peptide-ab16936.pdf

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Epigenetics and Nuclear Signaling Histones Variants
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Human Histone H1.2 peptide (ab16936)

  • Datasheet
Submit a review Q&A (1)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Purity: > 90% HPLC
  • Suitable for: Blocking

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Description

  • Product name

    Human Histone H1.2 peptide
    See all Histone H1.2 proteins and peptides
  • Purity

    > 90 % HPLC.

  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human

Associated products

  • Corresponding Antibody

    • Anti-Histone H1.2 antibody - ChIP Grade (ab4086)

Specifications

Our Abpromise guarantee covers the use of ab16936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Histone H1.2 antibody - ChIP Grade (ab4086)

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • H1 histone family member 2
    • H1.a
    • H12_HUMAN
    • H1F2
    • H1s-1
    • HIST1H1C
    • Histone 1 H1c
    • Histone cluster 1 H1c
    • Histone H1.2
    • Histone H1c
    • Histone H1d
    • Histone H1s-1
    • MGC3992
    see all
  • Function

    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • Sequence similarities

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P16403 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab16936? Please let us know so that we can cite the reference in this datasheet.

ab16936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

I'm working with your antibody anti Histone H1.2 (ab 4086)using WB technique with nice results. I obtain with my samples a band of aproximately 25 kDa as it is shown in your web. I would like to test the specificity of this band with your peptide Histone H1.2 (ab 16936)and I would like to know which is the protocol you could recommend me as in the web there are few details. Thank you.

Read More

Abcam community

Verified customer

Asked on Apr 25 2005

Answer

Thank you for your e-mail. We are very pleased to hear that you are satisfied with the antibody ab4086. I enclose our recommended protocol for blocking, please do not hesitate to contact us again if you need further information. In principle, a small volume of antibody (e.g., 1-5 ul) is first reacted with excess peptide (5-50 fold over the antibody; e.g., 1 ug antibody reacted with 5-50 ug peptide; exact amounts determined by titration) to neutralize it. The neutralized antibody can no longer subsequently bind to another antigen (a band of interest) or staining pattern. So the band(s)/staining that is competed by the antigen/peptide is supposedly specific. If more than one band disappears by peptide/antigen competition then those bands have the antigenic determinants and could be considered either fragments of the large antigen or multimer. 1. Determine the amount of antibody that is needed for 1 strip (e.g., 2 ml). For example, an antibody has given desired bands at 1:1000 dilution. So you will need 1 ul/ml antibody (2-ul antibody for 2-ml antibody solution). If an antibody were used at 1:5000 dilution then you would only need 0.2 ul/ml (use 2 ul of 1:10 dilution for better accuracy). 2. Take 2-ul antibody (or as needed) in 100 ul saline/PBS. Make 2 tubes. Add antigen/peptide solution (10-50 ug peptide or antigen added in 10-100 ul). Add same volume of saline/PBS (no peptide/antigen) to the other tube labeled as -peptide or No peptide. Mix gently. 3. Incubate both tubes at 37oC for 1-2 hrs or 2-24 hrs at 4oC. 4. Centrifuge the tubes for 15 min at 4oC in a microfuge (10-15000 rpm) to pellet any immune complexes. Carefully remove the supernatant. If no visible pellet is seen than just leave approx. 5-10 ul at the bottom to avoid disturbing invisible immune complexes. If you do not centrifuge the solution, it may give high background. 5. After centrifugation, make up the volume of supt. to 2 ml (or what is necessary depending upon the initial antibody taken) with buffer (PBS-Tween with or without BSA or milk or any buffer that was used initially). Use both antibodies (with and without antigen/peptide) for Western blotting or immunolocalization. 4. Observe the bands/staining that disappears. We have tested ab4086 specificity by mixing it at 1/500 with 1µg/ml of peptide Histone H1.2 ab16936.

Read More

Abcam Scientific Support

Answered on Apr 26 2005

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