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    human-histone-h2az-peptide-ab11681.pdf

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Epigenetics and Nuclear Signaling Histones Variants
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Human Histone H2A.Z peptide (ab11681)

  • Datasheet
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Western blot - Human Histone H2A.Z peptide (ab11681)

    Key features and details

    • Suitable for: Blocking

    Description

    • Product name

      Human Histone H2A.Z peptide
      See all Histone H2A.Z proteins and peptides
    • Accession

      P0C0S5
    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human

    Associated products

    • Corresponding Antibody

      • Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    Specifications

    Our Abpromise guarantee covers the use of ab11681 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

    • Form

      Liquid
    • Additional notes

      Synthetic peptide: C-SLIGKKGQQKT, corresponding to amino acids 116-126 of Human H2A.Z.

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names

      • H2A histone family member Z
      • H2A.z
      • H2A/z
      • H2afz
      • H2AZ
      • H2AZ_HUMAN
      • Histone H2A.Z
      • MGC117173
      see all
    • Function

      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • Sequence similarities

      Belongs to the histone H2A family.
    • Post-translational
      modifications

      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • Cellular localization

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P0C0S5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Human Histone H2A.Z peptide (ab11681)
      Western blot - Human Histone H2A.Z peptide (ab11681)
      All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/500 dilution

      Lanes 1 & 3 : Calf thymus histone lysate
      Lanes 2 & 4 : Calf thymus histone lysate with Human Histone H2A.Z peptide (ab11681) at 1 µg/ml

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size: 15 kDa
      why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab11681? Please let us know so that we can cite the reference in this datasheet.

    ab11681 has been referenced in 1 publication.

    • Kafer GR  et al. In Vitro Manipulation of Mammalian Preimplantation Embryos Can Alter Transcript Abundance of Histone Variants and Associated Factors. Cell Reprogram : (2011). WB . PubMed: 21827322

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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