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We are a research group very interested in purchasing your
Histone H3 peptide - acetyl K23 (ab48359).
Yet, in the website there is some confusing information and we need to
know exactly what will be sent when ordering:
There, it's written that the peptide has a length of 10 amino acids,
belonging to the 1-100 residues of the Human Histone H3 acetylated at
K23. Still, the Western-blot shows a 17kDa band, which cannot fit with a
10 amino acids length peptide.
1. What is the length of the peptide?
2. Do all the peptides contain the K23 position?
3. Do all the peptides have the K23 position acetylated?
Asked on Mar 29 2012
The peptide ab48359 was used to raise the antibody ab47813.
The peptide is indeed 10 residues long, located around the acetylation site K23. It was selected from within the region covering residues 1 to 100, but is not 100 residues long.
Yes, all peptides contain the K23 position and are acetylated at K23.
The WB image was done with the antibody ab47813 using HeLa Histone Preparation Nuclear Lysate - Butyrated. Lane 8 shows the result of the blocking experiment with the peptide ab48359. All other lanes show either the total lysate or blocking results with irrelevant peptides to show specificity of the antibody and peptide for acetyl K23.
Answered on Mar 29 2012