Human Histone H3 (citrulline R26) peptide (ab20631)



  • Nature
  • Amino Acid Sequence
    • Accession
    • Species
    • Modifications
      citrulline R26
    • Additional sequence information
      Also SwissProt ID: Q71DI3

Associated products


Our Abpromise guarantee covers the use of ab20631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Form
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • Cit Histone H3
    • Citrullated
    • citrullin
    • Citrullinated Histone H3
    • citrulline
    • H3 3 like sequence MH921
    • H3 3A
    • H3 histone
    • H3 histone family member E pseudogene
    • H3F3
    • HIST3H3
    • Histone H3 3 pseudogene
    • Histone H3 Citrullated
    • Histone H3.1
    • Histone H3.1t
    • Histone H3.2
    • Histone H3.3
    • Histone H3.3C
    • Histone H3.5
    see all
  • Relevance
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- modifications of histones, also called histone code, and nucleosome remodeling. P68431 and Q71DI3 are expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Q16695 is expressed in tesicular cells. Q6NXT2 is specifically expressed in the seminiferous tubules of testis and this Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes. P84243 is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Post-translational modification. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
  • Cellular localization


  • ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.

    Green and Orange lines: ELISA against immunising peptide (ab20631)
    Red and Mauve lines: ELISA against non-modified peptide (ab2623)


This product has been referenced in:
  • Zhang X  et al. Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor a target gene activation. Proc Natl Acad Sci U S A 109:13331-6 (2012). Read more (PubMed: 22853951) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your inquiry.

I can confirm that the peptide is in the following TK storage buffer:

30 mM HEPES, 150 mM NaCl, 2 mM EDTA, 0.001% Tween 20 at pH6.75


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