Other (Dot Blot) Abreview for Histone H3 peptide - di methyl K36

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Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.

No primary antibody directed towards H3H36me2 was used in this dot blot assay. This peptide was chosen to ensure there was no cross reactivity between the antibodies we are using on a regular basis. We have noticed very slight cross reactivity with this peptide using ab9050 anti-H3K36me3.
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H3 peptide - di methyl K36')
Primary antibody: Abcam primary antibody: Anti-Histone H3 (methylated) antibody - ChIP Grade (ab9050)
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

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Submitted Nov 18 2010

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