Application
Other
Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
Sample: Human Purified protein
Primary antibody (in addition to 'Histone H3 peptide - phospho S10')
Name: Non-Abcam antibody was used: anti-H2A.Z
Host species: Mouse
Clonality: Polyclonal
Dilution: 1/5000
Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000
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Submitted Oct 30 2010