Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644)

Overview

  • Product name
    Human Histone H3 (tri methyl K9, phospho S10) peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Modifications
      tri methyl K9, phospho S10

Specifications

Our Abpromise guarantee covers the use of ab15644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819), Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (ab14955)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • H3 histone
  • Relevance
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
  • Cellular localization
    Nuclear

Images

  • Rabbit polyclonal to Histone H3 (phospho S10, tri methyl K9) - ab5819, on histone preparations from HeLa cells. Primary antibody used at 1/500.

    Lanes 1, 3, 5, 7, 9 - control HeLa histones (0.5ug/lane)
    Lanes 2, 4, 6, 8, 10 - Colcemid-treated HeLa histones (0.5ug/lane)

    All blocking peptides are 1ug/lane

    Lanes 1 and 2: ab5819  
    Lanes 3 and 4: ab5819 + Histone H3 (Tri Me K9, phospho S10) peptide - ab15644
    Lanes 5 and 6: ab5819 + Histone H3 peptide (unmodified) - ab7228
    Lanes 7 and 8: ab5819 + Histone H3  (phospho S10) peptide - ab11477
    Lanes 9 and 10: ab5819 + Histone H3 (tri methyl K9) peptide - ab1773 

    Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721 1/5000.

    ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).

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References

ab15644 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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