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    human-histone-h4-tri-methyl-k20-peptide-ab17567.pdf

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Epigenetics and Nuclear Signaling Histones H4 Methylated
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Human Histone H4 (tri methyl K20) peptide (ab17567)

  • Datasheet
Reviews (1)Q&A (1)References (4)

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Western blot - Human Histone H4 (tri methyl K20) peptide (ab17567)

    Key features and details

    • Suitable for: Blocking

    Description

    • Product name

      Human Histone H4 (tri methyl K20) peptide
      See all Histone H4 proteins and peptides
    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human
      • Modifications

        tri methyl K20

    Associated products

    • Corresponding Antibody

      • Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)

    Specifications

    Our Abpromise guarantee covers the use of ab17567 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)

    • Form

      Lyophilized
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Information available upon request.

    General Info

    • Alternative names

      • dJ160A22.1
      • dJ160A22.2
      • dJ221C16.1
      • dJ221C16.9
      • FO108
      • H4
      • H4.k
      • H4/a
      • H4/b
      • H4/c
      • H4/d
      • H4/e
      • H4/g
      • H4/h
      • H4/I
      • H4/j
      • H4/k
      • H4/m
      • H4/n
      • H4/p
      • H4_HUMAN
      • H4F2
      • H4F2iii
      • H4F2iv
      • H4FA
      • H4FB
      • H4FC
      • H4FD
      • H4FE
      • H4FG
      • H4FH
      • H4FI
      • H4FJ
      • H4FK
      • H4FM
      • H4FN
      • H4M
      • HIST1H4A
      • HIST1H4B
      • HIST1H4C
      • HIST1H4D
      • HIST1H4E
      • HIST1H4F
      • HIST1H4H
      • HIST1H4I
      • HIST1H4J
      • HIST1H4K
      • HIST1H4L
      • HIST2H4
      • HIST2H4A
      • Hist4h4
      • Histone 1 H4a
      • Histone 1 H4b
      • Histone 1 H4c
      • Histone 1 H4d
      • Histone 1 H4e
      • Histone 1 H4f
      • Histone 1 H4h
      • Histone 1 H4i
      • Histone 1 H4j
      • Histone 1 H4k
      • Histone 1 H4l
      • Histone 2 H4a
      • histone 4 H4
      • Histone H4
      • MGC24116
      see all
    • Function

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similarities

      Belongs to the histone H4 family.
    • Post-translational
      modifications

      Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
      Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
      Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
      Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
      Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
      Sumoylated, which is associated with transcriptional repression.
    • Cellular localization

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P62805 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Human Histone H4 (tri methyl K20) peptide (ab17567)
      Western blot - Human Histone H4 (tri methyl K20) peptide (ab17567)

      ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab17567? Please let us know so that we can cite the reference in this datasheet.

    ab17567 has been referenced in 4 publications.

    • FitzGerald J  et al. Regulation of the DNA damage response and gene expression by the Dot1L histone methyltransferase and the 53Bp1 tumour suppressor. PLoS One 6:e14714 (2011). Blocking . PubMed: 21383990
    • Edwards CR  et al. Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing. Biochemistry 50:10473-83 (2011). PubMed: 21985125
    • Wang Y  et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). PubMed: 19250904
    • Heit R  et al. G2 histone methylation is required for the proper segregation of chromosomes. J Cell Sci 122:2957-68 (2009). PubMed: 19638412

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I have a few quick questions regarding a set of peptides we obtained and plan to use in pull-down experiments. The catalog numbers are ab14963, ab17043, ab14964, ab17567, corresponding to unmodified H4, H4K20me1, H4K20me2, and H4K20me3. - Do all these peptides have the same sequence? Is it possible to obtain that sequence? - what is the length of these petides? - do these peptides carry any sort of modification, i.e. do they have a cysteine at the N or C terminus, a biotin or a free accessible (not amidated or otherwise blocked) carboxy at the C terminus?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2012

    Answer

    Thank you for contacting Abcam regarding our Histone H4 peptides. The specific sequences of these peptides are considered proprietary and are not available on our website. The peptides are short, 8aa peptides and do contain a C terminal cysteine. If you would like additional information, please email our call our Scientific Support Department. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 10 2012

    Other (Dot Blot) Abreview for Histone H4 peptide - tri methyl K20

    Excellent
    Abreviews
    Abreviews
    Application
    Other - Do not use

    Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
    Sample: Human Purified protein

    Primary antibody (in addition to 'Histone H4 peptide - tri methyl K20')
    Primary antibody: None used

    Secondary antibody
    Secondary antibody: None used

    Abcam user community

    Verified customer

    Submitted Nov 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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