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  1. Link

    human-hladr-matched-antibody-pair-kit-ab222266.pdf

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Immunology Adaptive Immunity MHC Class II
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Recombinant

Human HLADR Matched Antibody Pair Kit (ab222266)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human HLADR standard curve
  • Sandwich ELISA - Human HLADR Matched Antibody Pair Kit (ab222266)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 31.25 pg/ml - 2000 pg/ml

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Overview

  • Product name

    Human HLADR Matched Antibody Pair Kit
    See all HLA-DR kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    4.57 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human HLADR Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human HLADR.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab223593. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 5 x 96 tests
    Human HLADR Capture Antibody 1 x 50µg
    Human HLADR Detector Antibody 1 x 12.5µg
    Human HLADR Lyophilized Protein 1 vial
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II
  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Target information above from: UniProt accession P01903 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • DASS-397D15.1
    • DR alpha chain
    • DR alpha chain precursor
    • DRA_HUMAN
    • DRB1
    • DRB4
    • FLJ51114
    • Histocompatibility antigen HLA DR alpha
    • Histocompatibility antigen HLA-DR alpha
    • HLA class II histocompatibility antigen
    • HLA class II histocompatibility antigen DR alpha chain
    • HLA DR1B
    • HLA DR3B
    • HLA DRA
    • HLA DRA1
    • HLA DRB1
    • HLA DRB3
    • HLA DRB4
    • HLA DRB5
    • HLA-DR histocompatibility type
    • HLA-DRA
    • HLADR4B
    • HLADRA1
    • HLADRB
    • Major histocompatibility complex class II DR alpha
    • Major histocompatibility complex class II DR beta 1
    • Major histocompatibility complex class II DR beta 3
    • Major histocompatibility complex class II DR beta 4
    • Major histocompatibility complex class II DR beta 5
    • MGC117330
    • MHC cell surface glycoprotein
    • MHC class II antigen DRA
    • MHC II
    • MLRW
    • OTTHUMP00000029406
    • OTTHUMP00000029407
    see all
  • Database links

    • Entrez Gene: 3122 Human
    • Omim: 142860 Human
    • SwissProt: P01903 Human
    • Unigene: 520048 Human

    Associated products

    • Alternative Versions

      • Human HLADR Antibody Pair - BSA and Azide free (ab241913)
    • Related Products

      • ELISA Accessory Pack (ab210905)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab222266 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.

    Images

    • Human HLADR standard curve
      Human HLADR standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human HLADR Matched Antibody Pair Kit (ab222266)
      Sandwich ELISA - Human HLADR Matched Antibody Pair Kit (ab222266)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab222266? Please let us know so that we can cite the reference in this datasheet.

    ab222266 has not yet been referenced specifically in any publications.

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