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I am interested in exploring the functional properties of the HMGB1 protein offered (ab18650) on mouse cells(PBMC). The description of your product states that it is derived from humans and this is what troubles me. An abreview shows data suggesting it might induce a response in a mouse cell line, but the effect (TLR4 surface expression) is (in my opinion) not the best way to tell that the protein induced a response. Do you have any other data from this protein that would confirm the stimulatory effect on mouse cells?. Would you recommend testing it?.
Asked on Dec 08 2011
Thank you for your enquiry. At this time the Abreview data is the only information we have suggesting ab18650 has functional properties. Do you feel TLR4 expression and exposure of cells to this region of HMGB1 are unrelated? If you have an alternative approach for using the peptide in a functional assay then it would certainly be interesting to see the results. I hope this helps to answer your questions. Please feel free to contact me again if you need anything further.
Answered on Dec 08 2011