Overview

  • Product name

    Human HO 1 ELISA Kit
    See all HO 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 30 < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall 30 < 10%
  • Sample type

    Serum, Tissue Extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.78 ng/ml
  • Range

    0.78 ng/ml - 25 ng/ml
  • Assay time

    2h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    Abcam’s HO-1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HO-1 in cell lysates and tissue extracts.

    A mouse monoclonal antibody specific for HO-1 is pre-coated on the wells of a plate. Standards or test samples are added to the wells, incubated and then washed. A HO-1 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

  • Notes

    Heme Oxygenase 1 (HO 1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has

    been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; “free” iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5’- or 3’- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO 1 or Hsp32, constitutive HO 2 that is abundant in the brain and testis, and HO 3 which is related to HO 2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well-known potent catalyst of lipid peroxidation and oxygen radical formation.

    The expression of HO 1 is highly responsive to all types of stimuli that cause oxidative stress and it is up regulated during exposure to oxidants, UV-A irradiation and a series of agents including cytokines, hormones, heme and heavy metals. HO 1 is a vital component of neuronal defense mechanisms and oxidative stress has been postulated to be the underlying basis for neuronal cell death in neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease. The expression of HO 1 is normally very low in the brain but increases markedly after heat shock, ischemia or glutathione depletion. Spatial distribution of HO 1 expression in AD brain is essentially identical to that of the pathogenic conformational changes of tau protein that is the major component of the intraneuronal lesion of AD, neurofibrillary tangles.

    HO 1 expression and tau expression may be regulated by oxidative stresses in a coordinated manner and play a pivitol role in the cytoprotection of neuronal cells. Plasma and cerebrospinal fluid HO 1 protein and lymphocyte HO 1 mRNA levels are decreased in subjects with sporadic AD relative to normal elderly controls suggesting that measurement of HO 1 may serve as a useful biological marker in early sporadic AD.

    Oxidative stress in the heart caused by ischemia and reperfusion has been shown to lead to cardiomyocyte death. An absence of HO 1 has detrimental consequences whereas overexpression of HO 1 plays a protective role in hypoperfusion and ischemia/reperfusion-induced myocardial injury. Under normal conditions, HO 1 is present at low levels in all organs except the spleen, but its expression is rapidly accelerated in response to pathophysiological conditions such as renal ischemia/reperfusion and cellular transformation. HO 1 overexpression exerts beneficial cytoprotective effects in a number of transplantation models, including antigen-independent

    ischemia/reperfusion injury, acute and chronic allograft rejection and xenotransplantation.

    The mechanisms by which HO 1 confers its protective effects are currently poorly understood but this area of investigation is active and rapidly evolving. The measurement of HO 1 in various cell types, tissues and bodily fluids may provide new insights into the physiological roles of HO 1 and may lead to monitoring HO 1 levels as a biomarker for therapeutic interventions or as an environmental biomarker in toxicology studies.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133064.

Protocols

References

This product has been referenced in:

  • Shono Y  et al. Characterization of a c-Rel Inhibitor That Mediates Anticancer Properties in Hematologic Malignancies by Blocking NF-?B-Controlled Oxidative Stress Responses. Cancer Res 76:377-89 (2016). ELISA ; Human . Read more (PubMed: 26744524) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

HO-1 for northern elephant seal

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used ab133064 for human and northern elephant seal serum. The antibody appears to work well. Heme oxygenase is well-conserved across species and the kit reacts as expected. We followed the protocol provided with no problems.

Dr. Daniel Cannon

Verified customer

Submitted May 15 2017

HO1 Human ELisa for serum samples

Good Excellent 5/5 (Ease of Use)
Abreviews
ABTRIAL-EBJ79
We have used 100ul undiluted serum for the assay, otherwise we have performed the assay as described in the "Instructions for Use".
The absorbance of all samples was within the points of the standard line, mainly between 1.56 and 6.25 ng/mL. Only 1 sample in the plate was under the lowest point of the standard curve. Samples from healthy subjects and patients that have been used in the assay were maintained without thawing, at -80ºC, from the collection to the day of analysis. The assay was made in duplicates and the variability for the duplicates (40 samples) was 2.8±3.8
Suggestion: To try lower samples volumes- prediluting the samples with Sample diluent to detemine minimal volumen requirement and assay sensitivity.

Abcam user community

Verified customer

Submitted Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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