Overview

  • Product name

    Human HO-1 ELISA Set (Reagents for 5 x 96 well plates, without plates)
    See all HO 1 kits
  • Sample type

    Cell Lysate, Microsomes
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.049 ng/ml
  • Range

    0.195 ng/ml - 12.5 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s HO-1 in vitro Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HO-1 in Biological fluids.

    A monoclonal antibody specific for HO-1 is coated onto 96-well plates. Samples and standards of HO-1 are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, HO-1 is recognized by the addition of a biotinylated monoclonal antibody specific for HO-1. After removal of excess biotinylated antibody, streptavidin-peroxidase is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of HO-1 in the samples

  • Notes

    Heme oxygenase (Hsp32) is the rate-limiting enzyme that breaks down heme to iron, carbon monoxide, and biliverdin, which is then metabolized to bilirubin by biliverdin reductase. In mammalians, heme oxygenase exists as two primary isoforms, the inducible isoform HO-1, and the constitutively expressed HO-2, both catalyzing the same reaction. HO-1 is expressed in erythrocyte and hemoglobin metabolizing tissues of the spleen, liver, and bone marrow, with localization to membranes of the ER, mitochondria, and caveolae . HO-1 expression is induced in response to an array of oxidative stress-inducing factors, including heat shock, heme accumulation, hypoxia, UV radiation, nitric oxide, cytokines, and heavy metals.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

Applications

Our Abpromise guarantee covers the use of ab133058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Dose-response curves from cell lysates diluted into assay buffer (using the MRD) were compared to the recombinant Human HO-1 standard curve. Parallelism indicates antibody-binding characteristics of the native and standard proteins are similar, allowing accurate determination of analyte.

  •  Representative Standard Curve using ab133058. 

Protocols

References

ab133058 has not yet been referenced specifically in any publications.

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