Human HSP Antibody Array - Membrane (9 Targets) (ab193661)

Overview

  • Product name

    Human HSP Antibody Array - Membrane (9 Targets)
  • Sample type

    Cell Lysate
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab193661 can be used for the simultaneous detection the relative levels of 9 Heat shock protein markers in cell lysates. 


    Targets: HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, GRP75, Ubiquitin+1, HSP10. 


    Learn more about membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotinylated Antibody Cocktail (C1) 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Human HSP Antibody Array Membranes (C1) 1 x 4 units 1 x 8 units 1 x 2 units
    Protease Inhibitor Cocktail 1 x 60µl 2 x 60µl 1 x 60µl

Applications

Our Abpromise guarantee covers the use of ab193661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Human HSP Antibody Array - Membrane (9 Targets) ab193661.These membranes were probed with conditioned media from HepG2 cell lines.

Protocols

References

ab193661 has not yet been referenced specifically in any publications.

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